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Synthesis, Characterization and Application of Porous Bioactive Glasses-Based Nanostructures in Bone Tissue Engineering

Aldhaher, Abdullah | 2023

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  1. Type of Document: Ph.D. Dissertation
  2. Language: Farsi
  3. Document No: 56111 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Bagherzadeh, Mojtaba; Baheiraei, Nafiseh
  7. Abstract:
  8. In the upcoming research, with the aim of bone tissue engineering and achieving a new structure, a scaffold based on polyhema (PHEMA) and gelatin (Gel), which are biocompatible polymers for bone tissue, was made and evaluated. Also, in order to improve the bioactivity and mechanical properties, bioactive glass alone (BG45S5) or together with strontium (BG-Sr) was used in the scaffold structure. and chemical by conducting FTIR, XRD, SEM, mechanical strength, bioactivity measurement, contact angle, water absorption and degradation tests. Biological investigations were done using mesenchymal stem cells derived from human bone marrow and with the help of MTT evaluations and SEM photography. The results obtained from this study confirmed the porous structure of scaffolds with interconnected pores in the range of 100-300 micrometers. The addition of bioactive glass increased the mechanical strength from 3.255±0.344 MPa for Gel-PHEMA scaffold to 21.964±0.564 kPa for Gel-PHEMA-BG scaffold. Conducting the bioactivity test by placing the samples in simulated body fluid (SBF) on day 7, confirmed the bioactivity of the fabricated scaffolds with the formation of hydroxyapatite crystals. After 7 weeks, the degradation rate of the samples showed a lower decrease in the samples containing BG-Sr, which confirms the strength and structural stability of this scaffold. The scaffolds did not cause cytotoxicity in the MTT test, and the ability of the prepared structure to provide a favorable environment to facilitate cell adhesion was confirmed through the SEM test. Based on the results obtained in this research, it can be claimed that Gel-PHEMA-BG-Sr scaffold is a suitable platform for bone tissue engineering
  9. Keywords:
  10. Tissue Engineering ; Bioactive Glass ; Strontium ; Bone Scaffold ; Bone Tissue Engineering ; Porous Nanostructure ; Biocompatible Polymer ; Polyhema (PHEMA)Biocompatible Polymer

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