Facile design of autogenous stimuli-responsive chitosan/hyaluronic acid nanoparticles for efficient small molecules to protein delivery

Sabourian, P ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1039/d0tb00772b
  3. Publisher: Royal Society of Chemistry , 2020
  4. Abstract:
  5. Easily assembled and biocompatible chitosan/hyaluronic acid nanoparticles with multiple stimuli-responsive ability are ideally suited for efficient delivery of therapeutic agents under specific endogenous triggers. We report a simple and versatile strategy to formulate oxidative stress and pH-responsive chitosan/hyaluronic acid nanocarriers with high encapsulation efficiencies of small drug molecules and nerve growth factor protein. This is achieved through invoking the dual role of a thioketal-based weak organic acid to disperse and functionalize low molecular weight chitosan in one-pot. Thioketal embedded chitosan/hyaluronic acid nanostructures respond to oxidative stress and show controlled release of quercetin, curcumin and NGF. Lowering the pH in the buffer solution led to higher quercetin release from NPs than at physiological pH, and mimicked the nanoparticle behavior in the environment of early to late endosomes. Curcumin and quercetin loaded NPs killed glioblastoma cells with high efficiency, and NGF-loaded nanoparticles retained biological activity of the protein and increased peripheral nerve outgrowth in explanted mouse dorsal root ganglia. This journal is © The Royal Society of Chemistry
  6. Keywords:
  7. Bioactivity ; Biocompatibility ; Chitosan ; Efficiency ; Flavonoids ; Molecules ; Nanoparticles ; pH ; Phenols ; Proteins ; Targeted drug delivery ; Dorsal root ganglia ; Encapsulation efficiency ; Glioblastoma cells ; Low molecular weight chitosans ; Nerve growth factor ; Stimuli-responsive ; Therapeutic agents ; Weak organic acids ; Controlled drug delivery
  8. Source: Journal of Materials Chemistry B ; Volume 8, Issue 32 , 2020 , Pages 7275-7287
  9. URL: https://pubs.rsc.org/en/content/articlelanding/2020/tb/d0tb00772b#!divAbstract