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Optimization of alginate-whey protein isolate microcapsules for survivability and release behavior of probiotic bacteria

Sajadi Dehkordi, S ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1007/s12010-019-03071-5
  3. Publisher: Springer , 2020
  4. Abstract:
  5. The present study aimed to improve the survivability of L. acidophilus encapsulated in alginate-whey protein isolate (AL-WPI) biocomposite under simulated gastric juice (SGJ) and simulated intestinal juice (SIJ). Microcapsules were prepared based on emulsification/internal gelation technique. Optimal compositions of AL and WPI and their ratio in the aqueous phase were evaluated based on minimizing mean diameter (MD) of the microcapsules and maximizing encapsulation efficiency (EE), survivability of cells under SGJ (Viability), and release of viable cells under SIJ (Release) using Box-Behnken experimental design. Optimal composition comprising 4.54% (w/v) AL, 10% (w/v) WPI, and 10% (v/v) AL-WPI gum in the aqueous phase was determined statistically. Physicochemical characteristics of the optimized matrix were investigated by SEM, FTIR, and XRD analysis to determine surface morphology, molecular bonds, and crystalline nature of such hydrocolloid. It could be concluded that the proposed biocomposite is a good promise for nutrients encapsulation in the food industry. © 2019, Springer Science+Business Media, LLC, part of Springer Nature
  6. Keywords:
  7. Box-Behnken design ; Lactobacillus acidophilus ; Sodium alginate ; Whey protein isolate ; Composite materials ; Emulsification ; Gelation ; Morphology ; Probiotics ; Proteins ; Surface morphology ; Box-Behnken experimental design ; Encapsulation efficiency ; Optimal composition ; Physicochemical characteristics ; Simulated gastric juice ; Microstructure ; Alginic acid ; Beta lactoglobulin ; Hydrogen ; Probiotic agent ; Whey protein ; Article ; Covalent bond ; Emulsion ; Encapsulation ; Food industry ; Fourier transform infrared spectroscopy ; Freeze drying ; Gelation ; Hydrocolloid ; Intestinal secretion ; Microcapsule ; Microencapsulation ; Nonhuman ; Particle size ; Scanning electron microscopy ; Stomach juice ; X ray diffraction
  8. Source: Applied Biochemistry and Biotechnology ; Volume 190, Issue 1 , 2020 , Pages 182-196
  9. URL: https://link.springer.com/article/10.1007/s12010-019-03071-5