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Curcumin-reduced graphene oxide sheets and their effects on human breast cancer cells

Hatamie, S ; Sharif University of Technology | 2015

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  1. Type of Document: Article
  2. DOI: 10.1016/j.msec.2015.05.077
  3. Publisher: Elsevier Ltd , 2015
  4. Abstract:
  5. Curcumin (as a natural reductant material) was utilized for green reduction and functionalization of chemically exfoliated graphene oxide (GO) sheets. The π-π attachment of the curcumin molecules onto the curcumin-reduced graphene oxide (rGO) sheets was confirmed by Raman and Fourier transform infrared spectroscopies. Zeta potential of the GO sheets decreased from about - 40 mV to - 20 mV, after the green reduction and functionalization. The probable cytotoxicity of the curcumin-rGO sheets was studied through their interactions with two human breast cancer cell lines (MDA-MB-231 and SKBR3 cell lines) and a normal cell line (mouse fibroblast L929 cell line). The curcumin-rGO sheet with concentrations < 70 μg/mL in the cell culture medium, not only exhibited no significant toxicity and/or cell morphological changes, but also caused some cell growths (~ 25% after 48 h incubation time). Nevertheless, at 70 μg/mL, initiation of some cell morphological changes was observed. At higher concentrations (e.g., 100 μg/mL), some slight cytotoxic effects (resulting in ~ 15 - 25% cell destruction) were detected by MTT assay. In addition, the interaction of the rGO sheets and cells resulted in apoptosis as well as morphological transformation of the cells (from elongated to roundup morphology). These results indicated the concentration-dependent toxicity of functionalized-rGO nanomaterials (here, curcumin-rGO) at the threshold concentration of ~ 100 μg/mL
  6. Keywords:
  7. Graphene ; Cell culture ; Cell death ; Cytology ; Cytotoxicity ; Diseases ; Fourier transform infrared spectroscopy ; Morphology ; Toxicity ; Cancer cells ; Cell viability ; Curcumin ; Human breast cancer cells ; Morphological transformations ; Reduced graphene oxides ; Reduced graphene oxides (RGO) ; Threshold concentrations ; Cells
  8. Source: Materials Science and Engineering C ; Volume 55 , 2015 , Pages 482-489 ; 09284931 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0928493115301296