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Synthesis and cyto-genotoxicity evaluation of graphene on mice spermatogonial stem cells

Hashemi, E ; Sharif University of Technology | 2016

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  1. Type of Document: Article
  2. DOI: 10.1016/j.colsurfb.2016.07.019
  3. Publisher: Elsevier , 2016
  4. Abstract:
  5. The present study analyzed the dose-dependent cyto- and genotoxicity of graphene oxide and reduced graphene oxide on spermatogonial stem cells (SSCs) for the first time. The results showed that graphene oxide significantly increased oxidative stress at concentrations of 100 and 400 μg/ml, while low concentrations did not have a significant effect. In addition, according to the MTT assay, the cell number decreased in high-concentration (100 and 400 μg/ml) graphene oxide-treated samples compared to untreated cells. However, a reduced graphene-treated sample demonstrated a significant increase in cell number. Moreover, microscopic analysis found high concentrations of graphene nanosheets in cell culture medium that reduced the number of colonies and colony forming cells. We conclude that a high concentration of graphene can be toxic to SSCs. However, such toxicity can be reduced by the surface modification of graphene nanomaterials. © 2016 Elsevier B.V
  6. Keywords:
  7. Cytotoxicity genotoxicity ; Graphene ; Spermatogonial stem cells ; Cell culture ; Cells ; Cytology ; Oxidative stress ; Stem cells ; Surface treatment ; Cell culture mediums ; Dose-dependent ; Genotoxicities ; Graphene nanosheets ; Graphene oxides ; Low concentrations ; Microscopic analysis ; Reduced graphene oxides ; Nanomaterial ; Adult stem cell ; Animal ; Apoptosis ; Bagg albino mouse ; Cell culture technique ; Chemistry ; DNA damage ; Drug effects ; Evaluation study ; Mouse ; Pathology ; Adult Germline Stem Cells ; Animals ; Apoptosis ; Cell Culture Techniques ; Cells, Cultured ; Graphite ; Male ; Mice ; Mice, Inbred BALB C ; Nanostructures
  8. Source: Colloids and Surfaces B: Biointerfaces ; Volume 146 , 2016 , Pages 770-776 ; 09277765 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0927776516305197