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Numerical analysis of a dielectrophoresis field-flow fractionation device for the separation of multiple cell types

Shamloo, A ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1002/jssc.201700325
  3. Abstract:
  4. In this study, a dielectrophoresis field-flow fractionation device was analyzed using a numerical simulation method and the behaviors of a set of different cells were investigated. By reducing the alternating current frequency of the electrodes from the value used in the original setup configuration and increasing the number of exit channels, total discrimination in cell trajectories and subsequent separation of four cell types were achieved. Cells were differentiated based on their size and dielectric response that are represented in their real part of Clausius–Mossotti factor at different frequencies. A number of novel designs were also proposed based on the original setup configuration. It was seen that by reducing the length of the main channel and the number of electrodes at low frequencies and not changing the inlet flow velocities, cell separation was still achieved successfully, although with a slightly larger electrode voltage. The shorter main channel decreased the residence time for the cells on the chip and also reduced the overall size of the device—these were improvements over the original design. The obtained results can be used to analyze other cell types by knowing their size and dielectric properties to design geometries that can ensure separation. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
  5. Keywords:
  6. Dielectrophoresis ; Dielectric properties ; Electrodes ; Electrophoresis ; Liquid chromatography ; Microfluidics ; Numerical methods ; Alternating current ; Dielectric response ; Different frequency ; Electrode voltage ; Multiple cell types ; Numerical simulation method ; Fractionation ; Cell destruction ; Cell differentiation ; Cell separation ; Cell size ; Controlled study ; Dielectric constant ; Electric conductivity ; Electric current ; Electric field ; Electrophoresis ; Erythrocyte ; Field flow fractionation ; Flow rate ; Hydrodynamics ; Leukocyte ; Mathematical computing ; Priority journal ; Shear stress ; T lymphocyte ; Thrombocyte ; Tumor cell ; Validation process ; Viscosity
  7. Source: Journal of Separation Science ; Volume 40, Issue 20 , 2017 , Pages 4067-4075 ; 16159306 (ISSN)
  8. URL: https://onlinelibrary.wiley.com/doi/pdf/10.1002/jssc.201700325