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Doxorubicin-conjugated D-glucosamine- and folate- bi-functionalised InP/ZnS quantum dots for cancer cells imaging and therapy

Ranjbar Navazi, Z ; Sharif University of Technology | 2018

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  1. Type of Document: Article
  2. DOI: 10.1080/1061186X.2017.1365876
  3. Publisher: Taylor and Francis Ltd , 2018
  4. Abstract:
  5. Nanoscaled quantum dots (QDs), with unique optical properties have been used for the development of theranostics. Here, InP/ZnS QDs were synthesised and functionalised with folate (QD-FA), D-glucosamine (QD-GA) or both (QD-FA-GA). The bi-functionalised QDs were further conjugated with doxorubicin (QD-FA-GA-DOX). Optimum Indium to fatty acid (In:MA) ratio was 1:3.5. Transmission electron microscopy (TEM) micrographs revealed spherical morphology for the QDs (11 nm). Energy-dispersive spectroscopy (EDS) spectrum confirmed the chemical composition of the QDs. MTT analysis in the OVCAR-3 cells treated with bare QDs, QD-FA, QD-GA, QD-FA-GA and QD-FA-GA-DOX (0.2 mg/mL of QDs) after 24 h indicated low toxicity for the bare QDs and functionalised QDs (about 80–90% cell viability). QD-FA-GA-DOX nanoparticles elicited toxicity in the cells. Cellular uptake of the engineered QDs were investigated in both folate receptor (FR)-positive OVCAR-3 cells and FR-negative A549 cells using fluorescence microscopy and FACS flow cytometry. The FA-functionalised QDs showed significantly higher uptake in the FR-positive OVCAR-3 cells, nonetheless the GA-functionalised QDs resulted in an indiscriminate uptake in both cell lines. In conclusion, our findings indicated that DOX-conjugated FA-armed QDs can be used as theranostics for simultaneous imaging and therapy of cancer. © 2017 Informa UK Limited, trading as Taylor & Francis Group
  6. Keywords:
  7. InP/ZnS quantum dots ; Solid tumours ; Targeted drug delivery ; Theranostics ; Doxorubicin ; Fatty acid ; Folate receptor ; Folic acid ; Glucosamine ; Indium ; Quantum dot ; A-549 cell line ; Article ; Cancer cell ; Chemical composition ; Controlled study ; Cytotoxicity ; Drug conjugation ; Drug release ; Energy dispersive X ray spectroscopy ; Female ; Flow cytometry ; Fluorescence activated cell sorting ; Fluorescence microscopy ; Human cell ; MTT assay ; OVCAR-3 cell line ; priority journal ; Transmission electron microscopy
  8. Source: Journal of Drug Targeting ; Volume 26, Issue 3 , 2018 , Pages 267-277 ; 1061186X (ISSN)
  9. URL: https://www.tandfonline.com/doi/abs/10.1080/1061186X.2017.1365876?journalCode=idrt20