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The role of polyethylene glycol size in chemical spectra, cytotoxicity, and release of pegylated nanoliposomal cisplatin

Shirzad, M ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1089/adt.2019.923
  3. Publisher: Mary Ann Liebert Inc , 2019
  4. Abstract:
  5. This study aimed to synthesize methoxy polyethylene glycol propionaldehyde (mPEG20,000-ALD) for the preparation of PEGylated nanoliposomal cisplatin. Nanocarriers such as liposomes are developed for a wide range of drug delivery systems. PEG with high molecular weight (Mw) is used to coat the liposomes. In this study, simulated Fourier transform infrared (FTIR) spectra of mPEG-ALD were obtained using density functional theory (DFT) calculations and then compared with actual FTIR spectrum of mPEG20,000-ALD (Mw = 20 kDa). We found that the intensity of C = O stretching vibration at 1,700 cm-1 related to the carbonyl functional group of mPEG20,000-ALD was very weak. The results of DFT calculations of mPEG-ALD showed that by increasing the Mw of mPEG-ALD, the intensity of C = O stretching vibration related to the carbonyl functional group of mPEG-ALD was decreased, so we concluded the hypothesis of decreasing the intensity of C = O stretching vibration at 1,700 cm-1 as a result of increasing the Mw of mPEG-ALD. In vitro release of cisplatin showed that the percentages of released cisplatin from PEGylated nanoliposomal cisplatin and free cisplatin were determined to be 46 ± 2% and 97 ± 3% after 35 h, respectively. Cytotoxicity of free cisplatin and PEGylated nanoliposomal cisplatin was evaluated and related half-maximal inhibitory concentration on human ovarian cancer cell line A2780CP was obtained to be 76.6 ± 3.1 and 46.6 ± 2.3 μg/mL, respectively. © 2019, Mary Ann Liebert, Inc
  6. Keywords:
  7. Cytotoxicity ; Density functional theory calculations ; Drug release ; Fourier transform infrared ; MPEG20,000-ALD ; PEGylated nanoliposomal cisplatin ; Carbonyl derivative ; Cisplatin ; Functional group ; Liposome ; Macrogol 20000 ; Nanocarrier ; Propionaldehyde ; A2780/CP70 cell line ; Calculation ; Controlled study ; Density functional theory ; Differential scanning calorimetry ; Drug cytotoxicity ; Drug efficacy ; Female ; Fourier transform infrared spectroscopy ; Human ; Human cell ; Hypothesis ; IC50 ; In vitro study ; Intermethod comparison ; Liposomal delivery ; Molecular weight ; Nanopharmaceutics ; Particle size ; PEGylation ; Photon correlation spectroscopy ; Simulation ; Stretching vibration ; Vibration ; Williamson reaction
  8. Source: Assay and Drug Development Technologies ; Volume 17, Issue 5 , 2019 , Pages 231-239 ; 1540658X (ISSN)
  9. URL: https://www.liebertpub.com/doi/abs/10.1089/adt.2019.923?journalCode=adt