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Design of a hybrid inertial and magnetophoretic microfluidic device for ctcs separation from blood

Nasiri, R ; Sharif University of Technology | 2021

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  1. Type of Document: Article
  2. DOI: 10.3390/mi12080877
  3. Publisher: MDPI AG , 2021
  4. Abstract:
  5. Circulating tumor cells (CTCs) isolation from a blood sample plays an important role in cancer diagnosis and treatment. Microfluidics offers a great potential for cancer cell separation from the blood. Among the microfluidic-based methods for CTC separation, the inertial method as a passive method and magnetic method as an active method are two efficient well-established methods. Here, we investigated the combination of these two methods to separate CTCs from a blood sample in a single chip. Firstly, numerical simulations were performed to analyze the fluid flow within the proposed channel, and the particle trajectories within the inertial cell separation unit were investigated to determine/predict the particle trajectories within the inertial channel in the presence of fluid dynamic forces. Then, the designed device was fabricated using the soft-lithography technique. Later, the CTCs were conjugated with magnetic nanoparticles and Ep-CAM antibodies to improve the magnetic susceptibility of the cells in the presence of a magnetic field by using neodymium permanent magnets of 0.51 T. A diluted blood sample containing nanoparticle-conjugated CTCs was injected into the device at different flow rates to analyze its performance. It was found that the flow rate of 1000 µL/min resulted in the highest recovery rate and purity of ~95% and ~93% for CTCs, respectively. © 2021 by the authors. Licensee MDPI, Basel, Switzerland
  6. Keywords:
  7. Diagnosis ; Diseases ; Flow of fluids ; Lithography ; Magnetic nanoparticles ; Magnetic susceptibility ; Magnetism ; Microfluidics ; Neodymium alloys ; Cancer diagnosis ; Circulating tumor cells ; Fluid dynamic forces ; Magnetic methods ; Micro-fluidic devices ; Microfluidic-based ; Particle trajectories ; Soft lithography ; Blood
  8. Source: Micromachines ; Volume 12, Issue 8 , 2021 ; 2072666X (ISSN)
  9. URL: https://www.mdpi.com/2072-666X/12/8/877