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Porphyrin molecules decorated on metal–organic frameworks for multi-functional biomedical applications

Rabiee, N ; Sharif University of Technology | 2021

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  1. Type of Document: Article
  2. DOI: 10.3390/biom11111714
  3. Publisher: MDPI , 2021
  4. Abstract:
  5. Metal–organic frameworks (MOFs) have been widely used as porous nanomaterials for different applications ranging from industrial to biomedicals. An unpredictable one-pot method is introduced to synthesize NH2-MIL-53 assisted by high-gravity in a greener media for the first time. Then, porphyrins were deployed to adorn the surface of MOF to increase the sensitivity of the prepared nanocomposite to the genetic materials and in-situ cellular protein structures. The hydrogen bond formation between genetic domains and the porphyrin’ nitrogen as well as the surface hydroxyl groups is equally probable and could be considered a milestone in chemical physics and physical chemistry for biomedical applications. In this context, the role of incorporating different forms of porphyrins, their relationship with the final surface morphology, and their drug/gene loading efficiency were investigated to provide a predictable pattern in regard to the previous works. The conceptual phenomenon was optimized to increase the interactions between the biomolecules and the substrate by reaching the limit of detection to 10 pM for the Anti-cas9 protein, 20 pM for the single-stranded DNA (ssDNA), below 10 pM for the single guide RNA (sgRNA) and also around 10 nM for recombinant SARS-CoV-2 spike antigen. Also, the MTT assay showed acceptable relative cell viability of more than 85% in most cases, even by increasing the dose of the prepared nanostructures. © 2021 by the authors. Licensee MDPI, Basel, Switzerland
  6. Keywords:
  7. Azithromycin ; CRISPR associated endonuclease Cas9 ; Doxorubicin ; Guide RNA ; Hemoglobin ; Hydroxyl group ; Metal organic framework ; Nanocomposite ; Nanomaterial ; Porphyrin ; Rosmarinus officinalis extract ; Single stranded DNA ; Biomedicine ; Cell viability ; Clustered regularly interspaced short palindromic repeat ; Confocal laser scanning microscopy ; Controlled study ; Cytotoxicity ; Drug sensitivity ; Fourier transform infrared spectroscopy ; Gene delivery system ; Human ; Human cell ; Immunization ; Limit of detection ; MCF-7 cell line ; MTT assay ; Nanotechnology ; Physical chemistry ; Rosemary ; Severe acute respiratory syndrome coronavirus 2 ; Spike ; Surface property ; X ray diffraction
  8. Source: Biomolecules ; Volume 11, Issue 11 , 2021 ; 2218273X (ISSN)
  9. URL: https://www.mdpi.com/2218-273X/11/11/1714