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Folic acid-decorated PH-responsive nanoniosomes with enhanced endocytosis for breast cancer therapy: in vitro studies

Rezaei, T ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.3389/fphar.2022.851242
  3. Publisher: Frontiers Media S.A , 2022
  4. Abstract:
  5. Breast cancer is the most common invasive cancer in women and the second leading cause of cancer death in women after lung cancer. The purpose of this study is a targeted delivery toward in vitro (on MCF7 and 4T1 breast cancer cell lines) through niosomes-based nanocarriers. To this end, different bioactive molecules, including hyaluronic acid (HA), folic acid (FA), and polyethylene glycol (PEG), were used and compared for surface modification of niosomes to enhance endocytosis. FA-functionalized niosomes (Nio/5-FU/FA) were able to increase cell cytotoxicity and reduce cell migration and invasion compared to PEG-functionalized niosomes (Nio/5-FU/PEG), and HA-functionalized niosomes (Nio/5-FU/HA) groups in MCF-7 and 4T1 cell lines. Although the Nio/5-FU/PEG and Nio/5-FU/HA demonstrated MCF7 cell uptake, the Nio/5-FU/FA exhibited the most preponderant endocytosis in pH 5.4. Remarkably, in this study 5-FU loaded niosomes (nonionic surfactant-based vesicles) were decorated with various bioactive molecules (FA, PEG, or HA) to compare their ability for breast cancer therapy. The fabricated nanoformulations were readily taken up by breast cancer cells (in vitro) and demonstrated sustained drug release characteristics, inducing cell apoptosis. Overall, the comprehensive comparison between different bioactive molecules-decorated nanoniosomes exhibited promising results in finding the best nano formulated candidates for targeted delivery of drugs for breast cancer therapy. Copyright © 2022 Rezaei, Rezaei, Karimifard, Mahmoudi Beram, Dakkali, Heydari, Afshari-Behbahanizadeh, Mostafavi, Bokov, Ansari, Farasati Far, Akbarzadeh and Chaiyasut
  6. Keywords:
  7. 5-FU ; Breast cancer ; Endocytosis ; Folic acid ; Hyaluronic acid ; Niosome ; Folic acid ; Hyaluronic acid ; Macrogol 2000 ; Niosome ; 4T1 cell line ; Animal cell ; Antineoplastic activity ; Apoptosis ; Controlled study ; Cytotoxicity ; Drug delivery system ; Drug formulation ; Endocytosis ; In vitro study ; MCF-7 cell line ; Mouse ; MTT assay ; Nanofabrication ; Nonhuman ; PH ; Response surface method ; Sustained drug release
  8. Source: Frontiers in Pharmacology ; Volume 13 , 2022 ; 16639812 (ISSN)
  9. URL: https://www.frontiersin.org/articles/10.3389/fphar.2022.851242/full