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Evaluating the effect of ultrasmall superparamagnetic iron oxide nanoparticles for a long-term magnetic cell labeling

Shanehsazzadeh, S ; Sharif University of Technology | 2013

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  1. Type of Document: Article
  2. DOI: 10.4103/0971-6203.106603
  3. Publisher: 2013
  4. Abstract:
  5. In order to evaluate the long-term viability, the iron content stability, and the labeling efficiency of mammalian cells using magnetic cell labeling; dextran-coated ultrasmall superparamagnetic iron oxide (USPIOs) nanoparticles with plain surfaces having a hydrodynamic size of 25 nm were used for this study. Tests were carried out in four groups each containing 5 flasks of 5.5 × 10 6 AD-293 embryonic kidney cells. The cell lines were incubated for 24 h using four different iron concentrations with and without protamine sulfate (Pro), washed with phosphate-buffered saline (PBS) and centrifuged three times to remove the unbounded USPIOs. Cell viability was also verified using USPIOs. There were no significant differences in the cell viability between the control group of cells and those groups with iron uptake at the specified iron concentrations. The average iron uptake ratio compared to that of the control group was (114 ± 1). The magnetic resonance images (MRI) at post-labeling day 1 and day 21 showed (75 ± 4)% and (22 ± 5)% signal decrements compared to that of the control, respectively. The Perl's Prussian blue test showed that 98% of the cells were labeled, and the iron concentration within the media did not affect the cell iron uptake. Magnetic cellular labeling with the USPIO-Pro complex had no short or medium term (3 weeks) toxic effects on AD-293 embryonic kidney cells
  6. Keywords:
  7. Magnetic resonance images ; Iron ; Phosphate buffered saline ; Protamine sulfate ; Ultrasmall superparamagnetic iron oxide ; Cell labeling ; Cell line ; Cell viability ; Cells ; Centrifugation ; Concentration (parameters) ; Controlled study ; Hydrodynamics ; Incubation time ; Iron transport ; Kidney cell ; Magnetic cell ; Magnetism ; Mammal cell ; Nuclear magnetic resonance imaging ; Particle size ; Surface property
  8. Source: Journal of Medical Physics ; Volume 38, Issue 1 , 2013 , Pages 34-40 ; 09716203 (ISSN)
  9. URL: http://www.jmp.org.in/article.asp?issn=0971-6203;year=2013;volume=38;issue=1;spage=34;epage=40;aulast=Shanehsazzadeh