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Response surface methodology as an approach to optimize growth medium of indigenous strain of Bacillus mycoides for production of biosurfactant

Najafi, A. R ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1109/ICBEE.2010.5650844
  3. Abstract:
  4. In this study, we have investigated the potential of a native bacterial strain isolated from an Iranian oil field for the production of biosurfactant. The bacterium was identified to be Bacillus mycoides by biochemical tests and 16S ribotyping. The biosurfactant, which was produced by this bacterium, was able to reduce the surface tension of media to 34 mN/m. Biosurfactant production was optimized by the combination of central composite design (CCD) and response surface methodology (RSM). The factor selected for optimization of growth conditions were pH, temperature, glucose and salinity concentrations. The empirical model developed through RSM in terms of effective operational factors mentioned above was found to be adequate to describe the biosurfactant production. Through the analysis, glucose and temperature were found to be the most significant factors, whereas pH and salinity had less effect within the ranges investigated. A maximum reduction in surface tension was obtained under the optimal conditions of 16.55 g/l glucose concentration, 39.03 °C, 55.05 g/l total salt concentration and medium pH 7.37
  5. Keywords:
  6. Bacillus mycoides ; Biosurfactant ; Oil ; RSM ; Surface tension ; Bacterial strains ; Bio surfactant ; Biochemical tests ; Biosurfactant production ; Central composite designs ; Empirical model ; Glucose concentration ; Growth conditions ; Growth medium ; Indigenous strain ; Operational factors ; Optimal conditions ; Response surface methodology ; Ribotyping ; Salt concentration ; Significant factors ; Bacteriology ; Biomolecules ; Environmental engineering ; Glucose ; Oil fields ; Optimization ; pH effects ; Salinity measurement ; Surface properties ; Surface active agents
  7. Source: ICBEE 2010 - 2010 2nd International Conference on Chemical, Biological and Environmental Engineering, Proceedings, 2 November 2010 through 4 November 2010 ; 2010 , Pages 146-152 ; 9781424487479 (ISBN)
  8. URL: http://ieeexplore.ieee.org/document/5650844