Loading...

Influence of Fe3O4 nanoparticles in hydroxyapatite scaffolds on proliferation of primary human fibroblast cells

Maleki Ghaleh, H ; Sharif University of Technology | 2016

1206 Viewed
  1. Type of Document: Article
  2. DOI: 10.1007/s11665-016-2086-4
  3. Publisher: Springer New York LLC , 2016
  4. Abstract:
  5. Modern techniques for expanding stem cells play a substantial role in tissue engineering: the raw material that facilitates regeneration of damaged tissues and treats diseases. The environmental conditions and bioprocessing methods are the primary determinants of the rate of cultured stem cell proliferation. Bioceramic scaffolds made of calcium phosphate are effective substrates for optimal cell proliferation. The present study investigates the effects of two bioceramic scaffolds on proliferating cells in culture media. One scaffold was made of hydroxyapatite and the other was a mixture of hydroxyapatite and ferromagnetic material (Fe3O4 nanoparticles). Disk-shaped (10 mm × 2 mm) samples of the two scaffolds were prepared. Primary human fibroblast proliferation was 1.8- and 2.5-fold faster, respectively, when cultured in the presence of hydroxyapatite or ferrous nanoparticle/hydroxyapatite mixtures. Optical microscopy images revealed that the increased proliferation was due to enhanced cell-cell contact. The presence of magnetic Fe3O4 nanoparticles in the ceramic scaffolds significantly increased cell proliferation compared to hydroxyapatite scaffolds and tissue culture polystyrene
  6. Keywords:
  7. Nanomaterials ; Biomaterials ; Cell culture ; Cell engineering ; Cell proliferation ; Cells ; Ceramic materials ; Cytology ; Ferromagnetic materials ; Fibroblasts ; Hydroxyapatite ; Magnetic materials ; Mixtures ; Nanomagnetics ; Nanoparticles ; Nanostructured materials ; Stem cells ; Tissue ; Tissue culture ; Tissue engineering ; Tissue regeneration ; Bioceramic scaffolds ; Cell-cell contact ; Ceramic matrix ; Ceramics ; Environmental conditions ; Human fibroblast cells ; Proliferating cells ; Tissue culture polystyrenes ; Scaffolds (biology)
  8. Source: Journal of Materials Engineering and Performance ; 2016 , Pages 1-9 ; 10599495 (ISSN)
  9. URL: https://link.springer.com/article/10.1007%2Fs11665-016-2086-4