Loading...

Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

Mahmoudifard, M ; Sharif University of Technology | 2018

1149 Viewed
  1. Type of Document: Article
  2. DOI: 10.1002/jbm.b.33923
  3. Publisher: John Wiley and Sons Inc , 2018
  4. Abstract:
  5. In the present study, the feasibility of electrospun polyethersolfone (PES) nanofibrous membrane as the solid substrate for microfluidic based immunoassays to enhance the density of immobilized antibody on the surface of membrane was assessed. Conversely, the efficacy of antibody immobilization was compared by two different strategies as 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) coupling chemistry and hydrophobic interaction. Compared to conventional immunoassays carried out in plates or gels, microfluidic based immunoassays grant a lot of advantages such as a consumption of little samples and reagents, shorter analysis time, and higher efficiency. Therefore, microfluidic immunoassays can be efficiently used as a point-of-care device in medical diagnosis. Surprisingly, we found the increase of specific surface areas of the microfluidic channels improve density of immobilized proteins and leads to higher signal strength. Anti-staphylococcus enterotoxin B (anti-SEB) was used as an analyte model to demonstrate the utility of our proposed platform. Fluorescent microscopy, Fourier transform infrared spectroscopic (FTIR), gas adsorption, contact angle, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), Uv-Vis spectrophotometer and atomic force microscopy (AFM) techniques were used to assess the efficacy of antibody immobilization on the surface. To understand dominant mechanism of protein immobilization, zeta potential measurement was also carried out and it was found electrostatic attraction play significant role in antibody immobilization running into micro- channels containing through EDC/NHS. Moreover, incorporation of nanofibrous membrane causes significant improvement in the signal detection of microfluidic based immunoassay. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1108–1120, 2018. © 2017 Wiley Periodicals, Inc
  6. Keywords:
  7. Immunoassay ; Microfluidic ; Nanofiber ; Antibodies ; Diagnosis ; Fluidic devices ; Fourier transform infrared spectroscopy ; Gas adsorption ; Hydrophobicity ; Immunology ; Nanofibers ; Proteins ; Antibody immobilization ; Electrostatic attractions ; Fourier transform infrareds ; Hydrophobic interactions ; Microfluidic immunoassay ; UV-VIS spectrophotometers ; Zeta potential measurements ; Antibody ; Immobilized antibody ; Polyethersolfone nanofiber ; Staphylococcus enterotoxin B antibody ; Unclassified drug ; Antigen binding ; Article ; Atomic force microscopy ; Contact angle ; Electrospinning ; Fluorescence microscopy ; Infrared spectroscopy ; Microfluidics ; Protein immobilization ; Scanning electron microscopy ; X ray photoelectron spectroscopy ; Zeta potential
  8. Source: Journal of Biomedical Materials Research - Part B Applied Biomaterials ; Volume 106, Issue 3 , April , 2018 , Pages 1108-1120 ; 15524973 (ISSN)
  9. URL: https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.b.33923