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Multifunctional tetracycline-loaded silica-coated core-shell magnetic nanoparticles: antibacterial, antibiofilm, and cytotoxic activities

Mazraeh, M ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1021/acsabm.2c00100
  3. Publisher: American Chemical Society , 2022
  4. Abstract:
  5. In the current study, the physicochemical and biological properties of tetracycline-loaded core-shell nanoparticles (Tet/Ni0.5Co0.5Fe2O4/SiO2 and Tet/CoFe2O4/SiO2) were investigated. The antibacterial activity of nanoparticles alone and in combination with tetracycline was investigated against a number of Gram-positive and Gram-negative bacteria for determining minimum inhibitory concentration (MIC) values. The MIC of Tet/Ni0.5Co0.5Fe2O4/SiO2 nanoparticles turned out to be significantly higher than that of Tet/CoFe2O4/SiO2 nanoparticles. Furthermore, Tet/Ni0.5Co0.5Fe2O4/SiO2 nanoparticles exhibited potent antibiofilm activity against pathogenic bacteria compared to Tet/CoFe2O4/SiO2 nanoparticles. The drug delivery potential of both carriers was assessed in vitro up to 124 h at different pH levels and it was found that the drug release rate was increased in acidic conditions. The cytotoxicity of nanoparticles was evaluated against a skin cancer cell line (melanoma A375) and a normal cell line (HFF). Our findings showed that Tet/Ni0.5Co0.5Fe2O4/SiO2 had greater cytotoxicity than CoFe2O4/SiO2 against the A375 cell line, whereas both synthesized nanoparticles had no significant cytotoxic effects on the normal cell line. Nonetheless, the biocompatibility of nanoparticles was assessed in vivo and the interaction of nanoparticles with the kidney was scrutinized up to 14 days. The overall results of the present study implied that the synthesized multifunctional magnetic nanoparticles with drug delivery potential, anticancer activity, and antibacterial activity are promising for biomedical applications. © 2022 American Chemical Society
  6. Keywords:
  7. Antibacterial ; Antibiofilm ; Antibiotic ; Biofilm gene ; Cytotoxicity ; Bacteria ; Cell culture ; Cells ; Controlled drug delivery ; Core shell nanoparticles ; Cytotoxicity ; Medical applications ; Nanomagnetics ; Oncology ; Physicochemical properties ; Shells (structures) ; Silica ; Silicon ; SiO2 nanoparticles ; Synthesis (chemical) ; Targeted drug delivery ; Anti-bacterial activity ; Antibacterials ; Antibiofilms ; Biofilm gene ; Cell lines ; Core-shell magnetic nanoparticles ; Minimum inhibitory concentration ; Silica coated ; SiO2 Nanoparticles ; Synthesised ; Biocompatibility ; Antiinfective agent ; Antineoplastic agent ; Magnetite nanoparticle ; Silicon dioxide ; Tetracycline ; Biofilm ; Chemistry ; Gram negative bacterium ; Gram positive bacterium ; Anti-Bacterial Agents ; Antineoplastic Agents ; Biofilms ; Gram-Negative Bacteria ; Gram-Positive Bacteria ; Magnetite Nanoparticles ; Silicon Dioxide ; Tetracycline
  8. Source: ACS Applied Bio Materials ; Volume 5, Issue 4 , 2022 , Pages 1731-1743 ; 25766422 (ISSN)
  9. URL: https://pubs.acs.org/doi/abs/10.1021/acsabm.2c00100