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Near infrared laser stimulation of human neural stem cells into neurons on graphene nanomesh semiconductors

Akhavan, O ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1016/j.colsurfb.2014.12.027
  3. Abstract:
  4. Reduced graphene oxide nanomeshes (rGONMs), as p-type semiconductors with band-gap energy of ~1. eV, were developed and applied in near infrared (NIR) laser stimulation of human neural stem cells (hNSCs) into neurons. The biocompatibility of the rGONMs in growth of hNSCs was found similar to that of the graphene oxide (GO) sheets. Proliferation of the hNSCs on the GONMs was assigned to the excess oxygen functional groups formed on edge defects of the GONMs, resulting in superhydrophilicity of the surface. Under NIR laser stimulation, the graphene layers (especially the rGONMs) exhibited significant cell differentiations, including more elongations of the cells and higher differentiation of neurons than glia. The higher hNSC differentiation on the rGONM than the reduced GO (rGO) was assigned to the stimulation effects of the low-energy photoexcited electrons injected from the rGONM semiconductors into the cells, while the high-energy photoelectrons of the rGO (as a zero band-gap semiconductor) could suppress the cell proliferation and/or even cause cell damages. Using conventional heating of the culture media up to ~43. °C (the temperature typically reached under the laser irradiation), no significant differentiation was observed in dark. This further confirmed the role of photoelectrons in the hNSC differentiation
  5. Keywords:
  6. Graphene nanomesh ; Nanostructures ; Neural differentiation ; NIR stimulation ; Stem cells ; Tissue engineering ; Biocompatibility ; Cell proliferation ; Cells ; Cytology ; Energy gap ; Functional groups ; Graphene ; Infrared devices ; Infrared lasers ; Neurons ; Photoelectrons ; Photons ; Semiconductor lasers ; Surface defects ; Band-gap semiconductors ; Cell differentiation ; High energy photoelectrons ; Neural differentiations ; Photoexcited electrons ; Reduced graphene oxides ; Cell engineering ; Graphene oxide ; Nanomesh ; Reduced graphene oxide nanomesh ; Unclassified drug ; Graphite ; Nanomaterial ; Oxide ; Article ; Cell damage ; Culture medium ; Electron ; Glia cell ; Heating ; Human ; Human cell ; Hydrophilicity ; Irradiation ; Near infrared laser stimulation ; Nerve cell ; Nerve cell differentiation ; Nerve cell growth ; Nerve cell stimulation ; Neural stem cell ; Semiconductor ; Cell culture ; Chemistry ; Laser ; Particle size ; Radiation response ; surface property ; Cells, cultured ; Humans ; Lasers ; Neural stem cells ; Oxides ; Semiconductors ; Surface Properties
  7. Source: Colloids and Surfaces B: Biointerfaces ; Volume 126 , 2015 , Pages 313-321 ; 09277765 (ISSN)
  8. URL: http://www.sciencedirect.com/science/article/pii/S0927776514007139