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Separation of phenolic glycolipids in mycobacterium bovis BCG by reversed-phase high performance liquid chromatography

Salimi, M ; Sharif University of Technology | 2009

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  1. Type of Document: Article
  2. Publisher: 2009
  3. Abstract:
  4. A crude phenolic glycolipid extract from Mycobacterium bovis BCG was fractionated by column chromatography. A reversed-phase high performance liquid chromatography (HPLC) method with UV detection at 275nm was developed for simultaneous detection and separation of phenolic glycolipids (PGLs) in Mycobacterium bovis BCG. This analysis provides a good resolution. Different solvent systems and columns for HPLC were compared. A system composed of acetonitrile-water in the ratio of 0→80% at a flow rate of 0.8 mL/min and C8 analytical column were found to be optimum for HPLC of the phenolic glycolipids. This simple method is therefore appropriate to purify these compounds present in M. bovis extract
  5. Keywords:
  6. HPLC-UV ; Mycobacterium ; Phenolic glycolipid ; Acetonitrile ; BCG vaccine ; Phenolic glycolipid ; Solvent ; Water ; Acetonitrile derivative ; Glycolipid ; Phenol derivative ; Article ; Flow rate ; Mycobacterium bovis ; Nonhuman ; Reversed phase high performance liquid chromatography ; Separation technique ; Ultraviolet radiation ; Chemistry ; Fractionation ; High performance liquid chromatography ; Isolation and purification ; Methodology ; Reproducibility ; Ultraviolet spectrophotometry ; Acetonitriles ; Chemical Fractionation ; Chromatography, High Pressure Liquid ; Glycolipids ; Mycobacterium bovis ; Phenols ; Reproducibility of Results ; Solvents ; Spectrophotometry, Ultraviolet ; Water
  7. Source: Pakistan Journal of Pharmaceutical Sciences ; Volume 22, Issue 2 , 2009 , Pages 145-149 ; 1011601X (ISSN)
  8. URL: https://www.ncbi.nlm.nih.gov/pubmed/19339223