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Gas chromatographic fingerprint analysis of secondary metabolites of Stachys lanata (Stachys byzantine C. Koch) combined with antioxidant activity modelling using multivariate chemometric methods

Aminfar, P ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1016/j.chroma.2019.06.002
  3. Publisher: Elsevier B.V , 2019
  4. Abstract:
  5. S. lanata has been traditionally used as a medicinal plant due to its various biological activities such as antioxidant activity. Therefore, identification and quality control studies of this plant are of great importance. To this end, gas chromatography (GC) combined with chemometrics was proposed for fingerprint analysis of S. lanata samples. This study sought to classify GC fingerprints of twenty-eight S. lanata samples from eight different regions of Iran and more importantly, to correlate fingerprints to the antioxidant activity to select S. lanata volatile antioxidant markers. S. lanata samples were classified into five and three classes using partial least squares-discriminant analysis (PLS-DA) according to their GC fingerprints and antioxidant peaks, respectively. The results of PLS regression (PLS-R) and variable importance in projection (VIP) showed that phenol, 2,4-bis (1,1-dimethylethyl)-, hexadecanoic acid- ethyl ester, vitamin E, Beta- sitosterol, and 1- monolinoleoylglycerol trimethylsily ether are volatile antioxidant markers of S. lanata samples. © 2019 Elsevier B.V
  6. Keywords:
  7. Antioxidant activity ; Chemometrics ; Partial least-squares regression ; Stachys lanata ; Discriminant analysis ; Gas chromatography ; Least squares approximations ; Metabolites ; Palmitic acid ; Anti-oxidant activities ; Chromatographic fingerprinting ; Partial least squares regression ; Quality control ; 1 monolinoleoylglycerol trimethylsily ether ; 2 hexadecanol ; 2,4 bis(1,1 dimethylethyl)phenol ; Alpha tocopherol ; Hexadecanoic acid ethyl ester ; Octacosane ; Sitosterol ; Stigmasta 3,5 dien 7 one ; Unclassified drug ; Antioxidant ; Chemometric analysis ; Controlled study ; Data analysis ; DPPH radical scavenging assay ; Iran ; Least square analysis ; Mass fragmentography ; Nonhuman ; Plant metabolism ; Priority journal ; Metabolism ; Principal component analysis ; Procedures ; Secondary metabolism ; Antioxidants ; Chromatography, Gas ; Discriminant Analysis ; Least-Squares Analysis ; Multivariate Analysis ; Stachys
  8. Source: Journal of Chromatography A ; Volume 1602 , 2019 , Pages 432-440 ; 00219673 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0021967319305850