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Folate conjugated hyaluronic acid coated alginate nanogels encapsulated oxaliplatin enhance antitumor and apoptosis efficacy on colorectal cancer cells (HT29 cell line)

Movahedi Shad, P ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1016/j.tiv.2019.104756
  3. Publisher: Elsevier Ltd , 2020
  4. Abstract:
  5. Oxaliplatin (OXA) has been widely used for treatment of colorectal cancer. In this study, to enhance antitumor and apoptosis efficacy, OXA was encapsulated in a novel folate conjugated hyaluronic acid coated alginate nanogels (F/HA/AL/OXA). The F/HA/AL/OXA nanogels were prepared by cross-linking process. The physico-chemical properties of F/HA/AL/OXA nanogels were characterized using scanning electron microscopy, transmission electron microscopy, fourier transform infrared spectroscopy, dynamic light scattering, and fluorescent spectrophotometry. The in-vitro antitumor activity of free OXA, AL, HA/AL, HA/AL/OXA and F/HA/AL/OXA nanogels were assessed using MTT assay against colorectal cancer cells (HT29 cell line). Finally, the effect of F/HA/AL/OXA nanogels on genes expression of Bax and Bcl2 was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) technique. The F/HA/AL/OXA nanogels were 200.3 nm in diameter and had a zeta potential of −22.0 mv. Antitumor activity of F/HA/AL/OXA nanogels on HT29 cell line indicated the highest antitumor activity as compared to free OXA and the empty nanogels. Compared to free OXA and the empty nanogels, the expression of Bax in HT29 cells treated with F/HA/AL/OXA nanogels was significantly increased along with suppression of Bcl-2 (p <. 01). In general, the present F/HA/AL/OXA nanogels are a promising carrier candidate for OXA to improve the anti-tumor activity. © 2019
  6. Keywords:
  7. Anti-tumor activity ; Apoptosis ; Colorectal cancer ; Folate- hyaluronic acid/alginate ; Hyaluronic acid ; Hyaluronic acid/alginate ; Oxaliplatin ; Alginic acid ; Folic acid ; Nanocarrier ; Nanogel ; Protein Bax ; Protein bcl 2 ; Unclassified drug ; Antineoplastic activity ; Bax gene ; Bcl2 gene ; Concentration response ; Controlled drug release ; Controlled study ; Cross linking ; Drug dosage form comparison ; Drug efficacy ; Flow cytometry ; Fourier transform infrared spectroscopy ; Gene expression ; HT-29 cell line ; Human cell ; In vitro study ; MTT assay ; Nanoencapsulation ; Particle size ; Photon correlation spectroscopy ; Physical chemistry ; Real time polymerase chain reaction ; Scanning electron microscopy ; Spectrofluorometry ; Transmission electron microscopy ; Zeta potential
  8. Source: Toxicology in Vitro ; Volume 65 , 2020
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0887233319306617#!