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Nanoscale characterization of the biomolecular corona by cryo-electron microscopy, cryo-electron tomography, and image simulation

Sheibani, S ; Sharif University of Technology | 2021

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  1. Type of Document: Article
  2. DOI: 10.1038/s41467-020-20884-9
  3. Publisher: Nature Research , 2021
  4. Abstract:
  5. The biological identity of nanoparticles (NPs) is established by their interactions with a wide range of biomolecules around their surfaces after exposure to biological media. Understanding the true nature of the biomolecular corona (BC) in its native state is, therefore, essential for its safe and efficient application in clinical settings. The fundamental challenge is to visualize the biomolecules within the corona and their relationship/association to the surface of the NPs. Using a synergistic application of cryo-electron microscopy, cryo-electron tomography, and three-dimensional reconstruction, we revealed the unique morphological details of the biomolecules and their distribution/association with the surface of polystyrene NPs at a nanoscale resolution. The analysis of the BC at a single NP level and its variability among NPs in the same sample, and the discovery of the presence of nonspecific biomolecules in plasma residues, enable more precise characterization of NPs, improving predictions of their safety and efficacies. © 2021, The Author(s)
  6. Keywords:
  7. Carbon ; Carboxylic acid ; Polystyrene ; Polystyrene derivative ; Detection method ; Electron microscopy ; Large eddy simulation ; Nanoparticle ; Polymer ; Simulation ; Tomography ; Accuracy ; Carboxylation ; Controlled study ; Electron tomography ; High resolution transmission electron microscopy ; Human ; Image analysis ; Nanoanalysis ; Pharmacokinetics ; Prediction ; Safety ; Surface property ; Chemistry ; Cryoelectron microscopy ; Procedures ; Reproducibility ; Three-dimensional imaging ; Transmission electron microscopy ; Ultrastructure ; Computer Simulation ; Electron Microscope Tomography ; Humans ; Imaging, Three-Dimensional ; Microscopy, Electron, Transmission ; Nanoparticles ; Plasma ; Polystyrenes ; Protein Corona ; Reproducibility of Results
  8. Source: Nature Communications ; Volume 12, Issue 1 , 2021 ; 20411723 (ISSN)
  9. URL: https://www.nature.com/articles/s41467-020-20884-9