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High-throughput micro-solid phase extraction on 96-well plate using dodecyl methacrylate-ethylen glycol dimethacrylate monolithic copolymer

Bagheri, H ; Sharif University of Technology | 2013

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  1. Type of Document: Article
  2. DOI: 10.1016/j.aca.2013.07.020
  3. Publisher: 2013
  4. Abstract:
  5. A novel high-throughput device based on 96-micro-solid phase extraction (96-μ-SPE) system was constructed for multiresidue determination of nine pesticides in aquatic samples. The extraction procedure was performed on a commercially available 96-well plate system. The extraction module consisted of 96 pieces of 1cm×3cm of cylindrically shaped stainless steel meshes. The prepared meshes were fixed in a home-made polytetrafluoroethylene-based constructed ninety-six holes block for possible simultaneous immersion of meshes into the center of individual wells. Dodecyl methacrylate and ethylene glycol dimethacrylate was copolymerized as a monolithic polymer and placed in the cylindrically shaped stainless steel meshes as extracting medium. A volume of 1mL of the aquatic sample was transferred into the 96-well plate and the 96-μ-SPE device was applied for the extraction of the selected pesticides. Subsequently, the extracted analytes were analyzed by gas chromatography-mass spectrometry. Influential parameters such as polymer synthesis conditions, sorbent-to-sorbent reproducibility, ionic strength and extraction time were optimized. Intra and inter-sorbent reproducibility on 96-μ-SPE device were evaluated and results revealed that extraction yields are rather similar. Limits of detection were below 4μgL-1 and the coefficient of determination was satisfactory (r2>0.99) for all the studied analytes. The developed method was successfully applied to the extraction and determination of the selected pesticides in surface water samples
  6. Keywords:
  7. Micro-solid phase extraction ; Monolithic polymer ; Pesticide multiresidue ; Monolithic polymers ; Multiresidue ; Copolymers ; Gas chromatography ; Ionic strength ; Pesticides ; Phase separation ; Polymers ; Sorption ; Extraction ; Copolymer ; Dodecyl methacrylate ; Ethylen glycol dimethacrylate ; Methacrylic acid derivative ; Pesticide ; Politef ; River water ; Stainless steel ; Surface water ; Unclassified drug ; Desorption ; Evaporation ; Limit of detection ; Mass fragmentography ; Polymerization ; Priority journal ; Reproducibility ; Solid phase extraction ; Water analysis ; 96-Well plate ; High-throughput ; Environmental Monitoring ; Methacrylates ; Microscopy, Electron, Scanning ; Pesticide Residues ; Rivers ; Water Pollutants, Chemical
  8. Source: Analytica Chimica Acta ; Volume 792 , August , 2013 , Pages 59-65 ; 00032670 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0003267013009665