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Niosomal delivery of simvastatin to MDA-MB-231 cancer cells

Akbarzadeh, I ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1080/03639045.2020.1810269
  3. Publisher: Taylor and Francis Ltd , 2020
  4. Abstract:
  5. Objective: The objective of this study was to use nano-niosomal formulations to deliver simvastatin as a poor-water soluble drug into breast cancer cells. Significance: Our study focused on the problem associated with poor water-soluble drugs which have significant biological activity in vivo. Methods: Different niosomal formulations of simvastatin were prepared and characterized in terms of morphology, size, encapsulation efficiency (EE), and release kinetic. Antiproliferative activity and the mechanism were assessed by quantitative real-time PCR and flow cytometry. Moreover, confocal microscopy was employed to analyze the cell uptake of simvastatin loaded niosomes to the cancerous cells. Results: Size, polydispersity index (PDI), and EE of the best formulation were obtained as 164.8 nm, 0.232, and 97%, respectively. The formulated simvastatin had a spherical shape and showed a slow release profile of the drug after 72 h. Stability data elucidated an increase in mean diameter and PDI which was lower for 4 °C than 25 °C. Confocal microscopy showed the localization of drug loaded niosomes in the cancer cells. The MTT assay revealed both free drug and drug loaded niosomes exhibited a dose-dependent cytotoxicity against breast cancer cells (MDA-MB-231 cells). Flow cytometry and qPCR analysis revealed drug loaded niosomes exert their cytotoxicity on cancerous cells via regulation of apoptotic and anti-apoptotic genes. Conclusion: The prepared niosomal simvastatin showed good physicochemical and biological properties than free drug. Our study suggests that niosomal delivery could be considered as a promising strategy for the delivery of poor water-soluble drugs to cancer cells. © 2020 Informa UK Limited, trading as Taylor & Francis Group
  6. Keywords:
  7. Breast cancer ; Drug delivery ; Niosome ; Simvastatin ; Solubility ; Annexin ; Antiproliferative activity ; Article ; Biological activity ; Cell viability ; Confocal microscopy ; Cytotoxicity ; Dispersity ; Drug formulation ; Drug release ; Encapsulation ; Experimental design ; Flow cytometry ; Fourier transform infrared spectroscopy ; Human ; Human cell ; IC50 ; In vitro study ; Kinetics ; MDA-MB-231 cell line ; MTT assay ; Real time polymerase chain reaction ; Response surface method ; Scanning electron microscopy
  8. Source: Drug Development and Industrial Pharmacy ; Volume 46, Issue 9 , 2020 , Pages 1535-1549
  9. URL: https://www.tandfonline.com/doi/abs/10.1080/03639045.2020.1810269?journalCode=iddi20