Loading...

CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin

Rabiee, N ; Sharif University of Technology | 2022

290 Viewed
  1. Type of Document: Article
  2. DOI: 10.1016/j.chemosphere.2022.135578
  3. Publisher: Elsevier Ltd , 2022
  4. Abstract:
  5. Overexpression of proteins/antigens and other gene-related sequences in the bodies could lead to significant mutations and refractory diseases. Detection and identification of assorted trace concentrations of such proteins/antigens and/or gene-related sequences remain challenging, affecting different pathogens and making viruses stronger. Correspondingly, coronavirus (SARS-CoV-2) mutations/alterations and spread could lead to overexpression of ssDNA and the related antigens in the population and brisk activity in gene-editing technologies in the treatment/detection may lead to the presence of pCRISPR in the blood. Therefore, the detection and evaluation of their trace concentrations are of critical importance. CaZnO-based nanoghosts (NGs) were synthesized with the assistance of a high-gravity technique at a 1,800 MHz field, capitalizing on the use of Rosmarinus officinalis leaf extract as the templating agent. A complete chemical, physical and biological investigation revealed that the synthesized NGs presented similar morphological features to the mesenchymal stem cells (MSCs), resulting in excellent biocompatibility, interaction with ssDNA- and/or pCRISPR-surface, through various chemical and physical mechanisms. This comprise the unprecedented synthesis of a fully inorganic nanostructure with behavior that is similar to MSCs. Furthermore, the endowed exceptional ability of inorganic NGs for detective sensing/folding of ssDNA and pCRISPR and recombinant SARS-CoV-2 spike antigen (RSCSA), along with in-situ hydrogen peroxide detection on the HEK-293 and HeLa cell lines, was discerned. On average, they displayed a high drug loading capacity of 55%, and the acceptable internalizations inside the HT-29 cell lines affirmed the anticipated MSCs-like behavior of these inorganic-NGs. © 2022 Elsevier Ltd
  6. Keywords:
  7. Gene delivery ; Nanoghosts ; SARS-CoV-2 ; ssDNA ; Antigens ; Biocompatibility ; Cell culture ; Controlled drug delivery ; Diseases ; Flowcharting ; Gene transfer ; Genes ; Lanthanum compounds ; Recombinant proteins ; Stem cells ; Targeted drug delivery ; In-situ biosensor ; Nanoghost ; Overexpressions ; PCRISPR ; Protein antigens ; SsDNA ; Synthesised ; Trace concentrations ; Coronavirus ; Calcium ; Doxorubicin ; Hydrogen peroxide ; Nanomaterial ; Recombinant antigen ; Rosmarinus officinalis extract ; Single stranded DNA ; Virus antigen ; Zinc oxide ; Antigen ; Detection method ; DNA ; Gene ; In situ measurement ; Nanoparticle ; Recombination ; Severe acute respiratory syndrome ; Bacillus cereus ; Cell viability ; Clustered regularly interspaced short palindromic repeat ; Controlled study ; Drug delivery system ; Gene editing ; Gene overexpression ; Gravity ; HEK293 cell line ; HeLa cell line ; HT-29 cell line ; Human ; Human cell ; In vitro study ; Internalization (cell) ; Mesenchymal stem cell ; Nonhuman ; Pseudomonas aeruginosa ; Rosemary ; Severe acute respiratory syndrome coronavirus 2 ; Synthesis ; Virus spike
  8. Source: Chemosphere ; Volume 306 , 2022 ; 00456535 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0045653522020719?via%3Dihub