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In vitro study of hydroxyapatite/polycaprolactone (HA/PCL) nanocomposite synthesized by an in situ sol-gel process

Rezaei, A ; Sharif University of Technology | 2013

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  1. Type of Document: Article
  2. DOI: 10.1016/j.msec.2012.09.004
  3. Publisher: 2013
  4. Abstract:
  5. Hydroxyapatite (HA) is the most substantial mineral constituent of a bone which has been extensively used in medicine as implantable materials, owing to its good biocompatibility, bioactivity high osteoconductive, and/or osteoinductive properties. Nevertheless, its mechanical property is not utmost appropriate for a bone substitution. Therefore, a composite consist of HA and a biodegradable polymer is usually prepared to generate an apt bone scaffold. In the present work polycaprolactone (PCL), a newly remarkable biocompatible and biodegradable polymer, was employed as a matrix and hydroxyapatite nanoparticles were used as a reinforcement element of the composite. HA/PCL nanocomposites were synthesized by a new in situ sol-gel process using calcium hydroxide and phosphoric acid precursors in the presence of Tetrahydrofuran (THF) as a solvent. Chemical and physical characteristics of the nanocomposite were studied by X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM) and Fourier transform infrared (FTIR) analyses. The results indicated that pure HA nanoparticles were well-incorporated and homogenously dispersed in the PCL matrix. It was found that the mechanical property of PCL was improved by addition of 20 wt.% HA nanoparticles. Furthermore, the biological property of nanocomposites was investigated under in vitro condition. For this purpose, HA/PCL scaffolds were prepared through a salt leaching process and immersed in a saturated simulated body fluid (SBF) after 3 and 7 days. It was found that a uniform layer of biomimetic HA could be deposited on the surface of HA/PCL scaffolds. Therefore, the prepared HA/PCL scaffolds showed good potential for bone tissue engineering and could be used for many clinical applications in orthopedic and maxillofacial surgery
  6. Keywords:
  7. In situ sol-gel process ; Nanocomposite ; Biological properties ; Bone scaffolds ; Bone substitution ; Bone tissue engineering ; Chemical and physical characteristics ; Clinical application ; Field emission scanning electron microscopy ; Fourier transform infrared ; Hydroxyapatite-polycaprolactone ; Implantable materials ; In-vitro ; Maxillofacial surgery ; Osteoconductive ; Osteoinductive properties ; Salt leaching ; Simulated body fluids ; Tetrahydrofurans ; Uniform layer ; Biocompatibility ; Biomimetics ; Bone ; Field emission microscopes ; Hydrated lime ; Mechanical properties ; Nanoparticles ; Phosphoric acid ; Polycaprolactone ; Scaffolds ; Scaffolds (biology) ; Sol-gel process ; X ray diffraction ; Organic solvents ; Hydroxyapatite ; polyester ; Chemistry ; Gel ; infrared spectroscopy ; Scanning electron microscopy ; Spectrometry ; Ultrastructure ; Durapatite ; Gels ; Microscopy, Electron, Scanning ; Polyesters ; Spectrometry, X-Ray Emission ; Spectroscopy, Fourier Transform Infrared
  8. Source: Materials Science and Engineering C ; Volume 33, Issue 1 , 2013 , Pages 390-396 ; 09284931 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0928493112004365