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Preparation and biological evaluation of radiolabeled-folate embedded superparamagnetic nanoparticles in wild-type rats

Jalilian, A. R ; Sharif University of Technology | 2011

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  1. Type of Document: Article
  2. DOI: 10.1007/s10967-010-0661-y
  3. Publisher: 2011
  4. Abstract:
  5. In this study, superparamagnetic iron oxide nanoparticles (SPION) embedded by folic acid (SPION-folate) were prepared by a modified co-precipitation method. The structure, size, morphology, magnetic property and relaxivity of the SPION-folate were characterized systematically by means of XRD, VSM, HRSEM and TEM and the interaction between folate and iron oxide (Fe3O 4) was characterized by FT-IR. The particle size was shown to be ≈5-10 nm. To ensure biocompatibility, the interaction of these SPION with mouse connective tissue cells (adhesive) was investigated using an MTT assay. Consequently, gallium-67 labeled nanoparticles ([67Ga]-SPION-folate) were prepared using 67Ga with a high labeling efficiency (over 96%, RTLC method) and they also showed an excellent stability at room temperature for at least 2 days and were evaluated for their biodistribution in normal rats up to 24 h compared with free Ga3+ cation and [67Ga]-SPION biodistribution. The biodistribution of the tracer among 3 other folate tracers were compared, showing lower liver uptake and higher blood circulation after 24 h leading to better bioavailability. The bone:muscle, kidney:muscle, lung:muscle, stomach:muscle ratios were 9.3, 9.32, 7.6 and 5.83 respectively. The developed folate-containing nano-system can be an interesting folate receptor tracer, capable of better cell membrane permeability while possessing paramagnetic properties for thermotherapy
  6. Keywords:
  7. 67Ga ; Biodistribution ; Folate ; Quality control ; SPION ; 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide ; Folic acid ; Gallium 67 ; Macrogol ; Superparamagnetic iron oxide nanoparticle ; Animal cell ; Animal experiment ; Animal tissue ; Biocompatibility ; Biological activity ; Bone ; Cell adhesion ; Cell interaction ; Controlled study ; Drug bioavailability ; Drug blood level ; Drug delivery system ; Drug determination ; Drug distribution ; Drug penetration ; Drug stability ; Drug structure ; Drug tissue level ; Drug uptake ; Infrared spectroscopy ; Isotope labeling ; Kidney ; Liver level ; Lung ; Molecular interaction ; Mouse ; Muscle ; Nanoanalysis ; Nanopharmaceutics ; Nonhuman ; Particle size ; Rat ; Room temperature ; Stomach ; Thin layer chromatography ; Tissue distribution ; Wild type
  8. Source: Journal of Radioanalytical and Nuclear Chemistry ; Volume 287, Issue 1 , January , 2011 , Pages 119-127 ; 02365731 (ISSN)
  9. URL: http://link.springer.com/article/10.1007%2Fs10967-010-0661-y