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Liquid-liquid-liquid microextraction followed by HPLC with UV detection for quantitation of ephedrine in urine

Bagheri, H ; Sharif University of Technology | 2008

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  1. Type of Document: Article
  2. DOI: 10.1002/jssc.200800193
  3. Publisher: 2008
  4. Abstract:
  5. Liquid-liquid-liquid microextraction (LLLME) in combination with HPLC and UV detection has been used as a sensitive method for the determination of ephedrine in urine samples. Extraction process was performed in a homemade total glass vial without using a Teflon ring, usually employed. Ephedrine was first extracted from 3.5 mL of urine sample (pH 12) into a microfilm of toluene/benzene (50:50). The analyte was subsequently back extracted into an acidic microdrop solution (pH 2) suspended in the organic phase. The extract was then injected into the HPLC system directly. An enrichment factor of 137 along with a good sample clean-up was obtained under the optimized conditions. The calibration curve showed linearity in the range of 0.01-50 mg/L with regression coefficient corresponding to 0.998. The LODs and LOQs, based on a S/N of 3 and 10, were 5 and 10 μg/L, respectively. The method was eventually applied for the determination of ephedrine in urine sample after oral administration of 5 mg single dose of drug. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA
  6. Keywords:
  7. Body fluids ; Chromatographic analysis ; Chromatography ; High performance liquid chromatography ; High pressure liquid chromatography ; Liquids ; Ultraviolet detectors ; Analyte ; Calibration curves ; Enrichment factors ; Ephedrine ; Extraction process ; Liquid-liquid-liquid microextraction ; Microdrop ; Optimized conditions ; Oral administrations ; Organic phase ; Regression coefficients ; Sensitive methods ; Single dose ; Urine sample ; UV detections ; Solvent extraction ; Benzene ; Toluene ; Adrenergic receptor stimulating agent ; Solvent ; Calibration ; Controlled study ; Drug determination ; Drug isolation ; Drug urine level ; Human experiment ; Liquid liquid extraction ; Normal human ; Priority journal ; Process optimization ; Quantitative analysis ; Single drug dose ; Ultraviolet spectroscopy ; Chemical structure ; Chemistry ; Doping ; Evaluation ; Fractionation ; Instrumentation ; Methodology ; Reproducibility ; Urine ; Chemical Fractionation ; Chromatography, High Pressure Liquid ; Doping in Sports ; Ephedrine ; Humans ; Molecular Structure ; Reproducibility of Results ; Solvents ; Sympathomimetics
  8. Source: Journal of Separation Science ; Volume 31, Issue 18 , 2008 , Pages 3212-3217 ; 16159306 (ISSN)
  9. URL: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/jssc.200800193