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Fabrication of biocompatible titanium scaffolds using space holder technique

Dezfuli, S. N ; Sharif University of Technology | 2012

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  1. Type of Document: Article
  2. DOI: 10.1007/s10856-012-4706-3
  3. Publisher: Springer , 2012
  4. Abstract:
  5. Open-pore titanium scaffolds were fabricated by sintering of compressed mixtures of TiH1.924 and urea. Spherical and irregular shaped space holders were used to investigate the effect of pore shape on cellular behavior. After removal of the space holder, the shape of the spacers was replicated to the pores. Average diameter of the pores was in the range of 300-600 lm. SEM images showed that titanium hydride resulted in higher surface roughness and larger micro porosities than pure titanium. In vitro evaluationswere carried out by using MTT assay, measuring alkaline phosphatase activity and alizarin red staining in flow perfusion bioreactor for cell culture. Observations revealed excellent attachment and proliferation of G-292 cells to the highly porous scaffolds fabricated with titanium hydride and urea of this research
  6. Keywords:
  7. Alizarin Red ; Alkaline phosphatase activity ; Average diameter ; Cellular behaviors ; Flow perfusion bioreactor ; In-vitro ; MTT assays ; Pore shape ; Porous scaffold ; SEM image ; Titanium hydride ; Titanium scaffolds ; Automobile manufacture ; Biological materials ; Cell culture ; Fabrication ; Hydrides ; Metabolism ; Phosphatases ; Sintering ; Surface roughness ; Titanium ; Scaffolds (biology) ; Alizarin red s ; Biomaterial ; Molecular scaffold ; Nitroblue tetrazolium ; Titanium hydride ; Unclassified drug ; Urea ; Bioreactor ; Cell adhesion ; Conference paper ; Enzyme activity ; Human ; Human cell ; Human cell culture ; In vitro study ; Morphology ; Nanofabrication ; Porosity ; Priority journal ; Procedures ; Space holder technique ; Alkaline Phosphatase ; Biocompatible Materials ; Cell Line ; Cell Line, Tumor ; Cell Proliferation ; Humans ; Microscopy, Electron, Scanning ; Tissue Scaffolds ; X-Ray Diffraction
  8. Source: Journal of Materials Science: Materials in Medicine ; Volume 23, Issue 10 , 2012 , Pages 2483-2488 ; 09574530 (ISSN)
  9. URL: http://link.springer.com/article/10.1007%2Fs10856-012-4706-3