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Smart liposomal drug delivery for treatment of oxidative stress model in human embryonic stem cell-derived retinal pigment epithelial cells

Behroozi, F ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijpharm.2018.05.056
  3. Abstract:
  4. Oxidative stress has been implicated in the progression of age-related macular degeneration (AMD). Treatment with antioxidants seems to delay progression of AMD. In this study, we suggested an antioxidant delivery system based on redox-sensitive liposome composed of phospholipids and a diselenide centered alkyl chain. Dynamic light scattering assessment indicated that the liposomes had an average size of 140 nm with a polydispersity index below 0.2. The percentage of encapsulation efficiency of the liposomes was calculated by high-performance liquid chromatography. The carriers were loaded with N-acetyl cysteine as a model antioxidant drug. We demonstrated responsiveness of the nanocarrier and its efficiency in drug delivery in an oxidative stress model of human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cells. The modeled cells treated with diselenide containing liposomes loaded with 10 mM NAC, showed a better therapeutic effect with a cell metabolic activity of 90%, which was significantly higher compared to insensitive liposomes or NAC treated groups (P < 0.05). In addition, the expression of oxidative-sensitive gene markers in diselenide containing liposomes groups were improved. Our results demonstrated fabricated smart liposomes opens new opportunity for targeted treatment of retinal degeneration. © 2018 Elsevier B.V
  5. Keywords:
  6. Age macular degeneration ; Diselenide ; Drug delivery ; Liposome ; Redox sensitive ; Nanocarrier ; Antioxidant ; Phospholipid ; Reactive oxygen metabolite ; Selenium ; Age related macular degeneration ; Controlled study ; Cytotoxicity assay ; Drug release ; Embryo ; Human ; Human cell ; In vitro study ; Liposomal delivery ; Oxidation reduction reaction ; Priority journal ; Retina pigment cell ; Zeta potential ; Cell culture ; Cytology ; Drug effect ; Epithelium cell ; Metabolism ; Acetylcysteine ; Antioxidants ; Cell survival ; Cells, cultured ; Drug liberation ; Epithelial cells ; Human embryonic stem cells ; Humans ; Liposomes ; Oxidative stress ; Phospholipids ; Reactive oxygen species ; Retinal pigment epithelium
  7. Source: International Journal of Pharmaceutics ; Volume 548, Issue 1 , 2018 , Pages 62-72 ; 03785173 (ISSN)
  8. URL: https://www.sciencedirect.com/science/article/pii/S0378517318303478?via%3Dihub