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Bottom-up synthesis of nitrogen and oxygen co-decorated carbon quantum dots with enhanced DNA plasmid expression

Yadegari, A ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1016/j.colsurfb.2019.110543
  3. Publisher: Elsevier B.V , 2019
  4. Abstract:
  5. In this paper, a bottom-up hydrothermal route is reported for the synthesis of oxygen and nitrogen co-decorated carbon quantum dots (CQDs) using ammonium hydrogen citrate (AHC) as a single precursor. DLS data approved the formation of 4.0 nm (average size) CQDs. XRD pattern shows the interlayer spacing (002) of 3.5 Å for CQDs, which is exactly the same as that of crystalline graphite. XPS and FTIR spectra verified the formation of oxygen and nitrogen functional groups on the CQDs surface. Co-decorated carboxyl, hydroxyl and amine groups on the CQDs surfaces make them as promising polyelectrolyte for gene delivery. Toxicity assay showed a survival rate of 70% under different incubation times and up to 500 μg/mL. The highly water-soluble, stable fluorescence and low toxic CQDs increased the gene expression of DNA plasmid in E. coli bacteria 4-fold more than the control group. © 2019 Elsevier B.V
  6. Keywords:
  7. Biocompatible ; Carbon quantum dot ; Gene delivery ; Transformation ; Biocompatibility ; Carbon ; DNA ; Fourier transform infrared spectroscopy ; Gene expression ; Gene transfer ; Hydrothermal synthesis ; Nanocrystals ; Nitrogen ; Biocompatible ; Bottom-up synthesis ; Crystalline graphite ; Hydrothermal routes ; Interlayer spacings ; Oxygen and nitrogens ; Semiconductor quantum dots ; Ammonium derivative ; Ammonium hydrogen citric acid ; Citric acid ; Graphite ; Oxygen ; Plasmid DNA ; Quantum dot ; Uunclassified drug ; Oxygen ; Polyelectrolyte ; Quaternary ammonium derivative ; Article ; Controlled study ; Gene delivery system ; Incubation time ; Nonhuman ; Photoluminescence ; Polymerase chain reaction ; Priority journal ; Synthesis ; Transmission electron microscopy ; X ray diffraction ; X ray photoemission spectroscopy ; Chemistry ; Drug effect ; Genetics ; Particle size ; Plasmid ; Surface property ; Citrates ; Escherichia coli ; Gene Transfer Techniques ; Nitrogen ; Plasmids ; Polyelectrolytes ; Quantum Dots ; Quaternary Ammonium Compounds ; Surface Properties
  8. Source: Colloids and Surfaces B: Biointerfaces ; Volume 184 , 2019 ; 09277765 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0927776519306873