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A dual functional chondro-inductive chitosan thermogel with high shear modulus and sustained drug release for cartilage tissue engineering

Dehghan Baniani, D ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijbiomac.2022.02.115
  3. Publisher: Elsevier B.V , 2022
  4. Abstract:
  5. We report a chitosan-based nanocomposite thermogel with superior shear modulus resembling that of cartilage and dual pro-chondrogenic and anti-inflammatory functions. Two therapeutic agents, kartogenin (KGN) and diclofenac sodium (DS), are employed to promote chondrogenesis of stem cells and suppress inflammation, respectively. To extend the release time in a controlled manner, KGN is encapsulated in the uniform-sized starch microspheres and DS is loaded into the halloysite nanotubes. Both drug carriers are doped into the maleimide-modified chitosan hydrogel to produce a shear modulus of 167 ± 5 kPa that is comparable to that of articular cartilage (50–250 kPa). Owing to the hydrogel injectability and relatively suitable gelation time (5 ± 0.5 min) at 37 °C, this system potentially constitutes a manageable platform for clinical practice. Moreover, sustained linear drug release for over a month boosts chondro-differentiation of stem cells to eliminate the necessity for multiple administrations. Considering virtues such as thermogel strength and ability to co-deliver anti-inflammatory and chondro-inductive biomolecules continuously, the materials and strategy have promising potential in functional cartilage tissue engineering. © 2022 Elsevier B.V
  6. Keywords:
  7. Cartilage tissue engineering ; Chitosan hydrogel ; Kartogenin ; Starch microspheres ; Agents affecting metabolism ; Chitosan nanocomposite thermogel ; Diclofenac ; Hydrogel ; Maleimide ; Nanogel ; Nanotube ; Starch microsphere ; Unclassified drug ; Antiinflammatory activity ; Biocompatibility ; Cartilage ; Chemical modification ; Clinical practice ; Controlled study ; Gelation ; Gelation time ; Human ; Human cell ; In vitro study ; Injectability ; Mesenchymal stem cell ; Nanoencapsulation ; Physical parameters ; Shear modulus ; Strength ; Sustained drug release ; Articular cartilage ; Drug release ; Cartilage, Articular ; Cell Differentiation ; Chitosan ; Chondrogenesis ; Drug Liberation ; Hydrogels ; Mesenchymal Stem Cells ; Tissue engineering
  8. Source: International Journal of Biological Macromolecules ; Volume 205 , 2022 , Pages 638-650 ; 01418130 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0141813022003610