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Multilayered mesoporous composite nanostructures for highly sensitive label-free quantification of cardiac troponin-i

Saeidi, M ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.3390/bios12050337
  3. Publisher: MDPI , 2022
  4. Abstract:
  5. Cardiac troponin-I (cTnI) is a well-known biomarker for the diagnosis and control of acute myocardial infarction in clinical practice. To improve the accuracy and reliability of cTnI electrochemical immunosensors, we propose a multilayer nanostructure consisting of Fe3O4-COOH labeled anti-cTnI monoclonal antibody (Fe3O4-COOH-Ab1 ) and anti-cTnI polyclonal antibody (Ab2 ) conjugated on Au-Ag nanoparticles (NPs) decorated on a metal–organic framework (Au-Ag@ZIF-67-Ab2 ). In this design, Fe3O4-COOH was used for separation of cTnI in specimens and signal amplification, hierarchical porous ZIF-67 extremely enhanced the specific surface area, and Au-Ag NPs synergically promoted the conductivity and sensitivity. They were additionally employed as an immobilization platform to enhance antibody loading. Electron microscopy images indicated that Ag-Au NPs with an average diameter of 1.9 ± 0.5 nm were uniformly decorated on plate-like ZIF-67 particles (with average size of 690 nm) without any agglomeration. Several electrochemical assays were implemented to precisely evaluate the immunosensor performance. The square wave voltammetry technique exhibited the best performance with a sensitivity of 0.98 mA mL cm−2 ng−1 and a detection limit of 0.047 pg mL−1 in the linear range of 0.04 to 8 ng mL−1 . © 2022 by the authors. Licensee MDPI, Basel, Switzerland
  6. Keywords:
  7. Cardiac troponin-I ; Electrochemical immunosensors ; Gold and silver decorated ZIF-67 ; Heart attack risk ; Cardiac troponin I ; Cardiac troponin I monoclonal antibody ; Cardiac troponin I polyclonal antibody ; Gold nanoparticle ; Iron oxide nanoparticle ; Monoclonal antibody ; Nanomaterial ; Polyclonal antibody ; Silver nanoparticle ; Unclassified drug ; Immobilized antibody ; Metal nanoparticle ; Accuracy ; Cardiovascular risk ; Conductance ; Controlled study ; Electrochemical analysis ; Electron microscopy ; Heart infarction ; Immobilization ; Limit of detection ; Particle size ; Protein analysis ; Reliability ; Sensitivity analysis ; Square wave voltammetry ; Surface area ; Chemistry ; Genetic procedures ; Procedures ; Reproducibility ; Antibodies, Immobilized ; Biosensing Techniques ; Electrochemical Techniques ; Gold ; Immunoassay ; Metal Nanoparticles ; Reproducibility of Results ; Silver ; Troponin I
  8. Source: Biosensors ; Volume 12, Issue 5 , 2022 ; 20796374 (ISSN)
  9. URL: https://www.mdpi.com/2079-6374/12/5/337