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Improving bactericidal performance of implant composite coatings by synergism between Melittin and tetracycline

Zarghami, V ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1007/s10856-022-06666-3
  3. Publisher: Springer , 2022
  4. Abstract:
  5. Methicillin resistance Staphylococcus aureus bacteria (MRSA) are serious hazards of bone implants. The present study was aimed to use the potential synergistic effects of Melittin and tetracycline to prevent MRSA associated bone implant infection. Chitosan/bioactive glass nanoparticles/tetracycline composite coatings were deposited on hydrothermally etched titanium substrate. Melittin was then coated on composite coatings by drop casting method. The surfaces were analyzed by FTIR, XRD, and SEM instruments. Tetracycline in coatings revealed multifunctional behaviors include bone regeneration and antibacterial activity. Releasing ALP enzyme from MC3T3 cells increased by tetracycline, so it is suitable candidate as osteoinductive and antibacterial agent in orthopedic implants coatings. Melittin increased the proliferation of MC3T3 cells. Composite coatings with combination of tetracycline and Melittin eradicate all MRSA bacteria, while coatings with one of them could no t eradicate all of the bacteria. In conclusion, chitosan/bioactive glass/tetracycline/Melittin coating can be suggested as a multifunctional bone implant coating because of its osteogenic and promising antibacterial activity. [Figure not available: see fulltext.] © 2022, The Author(s)
  6. Keywords:
  7. Composite coatings ; Enzyme activity ; Glass ; Peptides ; Anti-bacterial activity ; Bone implant ; Composites coating ; Hydrothermally ; Implant infections ; Melittin ; Methicillin ; Performance ; Staphylococcus aureus ; Synergistic effect ; Bacteria ; Alkaline phosphatase ; Nanoparticle ; Antiinfective agent ; Biocompatible coated material ; Tetracycline ; Antibacterial activity ; Bacterial clearance ; Bactericidal activity ; Biocompatibility ; Bone cell ; Bone regeneration ; Cell differentiation ; Cell proliferation ; Coating (procedure) ; Controlled study ; DNA content ; Drug potentiation ; Field emission scanning electron microscopy ; Fourier transform infrared spectroscopy ; Implant infection ; MC3T3 cell line ; Methicillin resistance ; Methicillin resistant Staphylococcus aureus infection ; Nonhuman ; Surface property ; X ray diffraction ; Methicillin resistant Staphylococcus aureus ; Anti-Bacterial Agents ; Chitosan ; Coated Materials, Biocompatible ; Melitten ; Methicillin-Resistant Staphylococcus aureus ; Tetracycline ; Titanium
  8. Source: Journal of Materials Science: Materials in Medicine ; Volume 33, Issue 6 , 2022 ; 09574530 (ISSN)
  9. URL: https://pubmed.ncbi.nlm.nih.gov/35596852