Loading...

Formulation and characterization of poly (ethylene glycol)-coated core-shell methionine magnetic nanoparticles as a carrier for naproxen delivery: growth inhibition of cancer cells

Yeganeh, F. E ; Sharif University of Technology | 2022

146 Viewed
  1. Type of Document: Article
  2. DOI: 10.3390/cancers14071797
  3. Publisher: MDPI , 2022
  4. Abstract:
  5. An efficient and selective drug delivery vehicle for cancer cells can remarkably improve therapeutic approaches. In this study, we focused on the synthesis and characterization of magnetic Ni1−x Cox Fe2 O4 nanoparticles (NPs) coated with two layers of methionine and polyethylene glycol to increase the loading capacity and lower toxicity to serve as an efficient drug car-rier. Ni1−x Cox Fe2 O4 @Methionine@PEG NPs were synthesized by a reflux method then charac-terized by FTIR, XRD, FESEM, TEM, and VSM. Naproxen was used as a model drug and its loading and release in the vehicles were evaluated. The results for loading efficiency showed 1 mg of Ni1−x Cox Fe2 O4 @Methionine@PEG NPs could load 0.51 mg of the naproxen. Interest-ingly, Ni1−x Cox Fe2 O4 @Methionine@PEG showed a gradual release of the drug, achieving a time-release up to 5 days, and demonstrated that a pH 5 release of the drug was about 20% higher than Ni1−x Cox Fe2 O4 @Methionine NPs, which could enhance the intracellular drug release following endocytosis. At pH 7.4, the release of the drug was slower than Ni1−x Cox Fe2 O4 @Methionine NPs; demonstrating the potential to minimize the adverse effects of anticancer drugs on normal tissues. Moreover, naproxen loaded onto the Ni1−x Cox Fe2 O4 @Methionine@PEG NPs for breast cancer cell lines MDA-MB-231 and MCF-7 showed more significant cell death than the free drug, which was measured by an MTT assay. When comparing both cancer cells, we demonstrated that naproxen loaded onto the Ni1−x Cox Fe2 O4 @Methionine@PEG NPs exhibited greater cell death effects on the MCF-7 cells compared with the MDA-MB-231 cells. The results of the hemolysis test also showed good hemocompatibility. The results indicated that the prepared magnetic nanocarrier could be suitable for controlled anticancer drug delivery. © 2022 by the authors. Licensee MDPI, Basel, Switzerland
  6. Keywords:
  7. Cell line ; Drug delivery ; Ni1−x Cox Fe2 O4 NPs ; PEGylating ; Antineoplastic agent ; Macrogol ; Macrogol 6000 ; Magnetic nanoparticle ; Methionine ; Naproxen ; Breast cancer cell line ; Cancer cell ; Cancer inhibition ; Cell death ; Cell survival rate ; Controlled study ; Cytotoxicity ; Drug delivery system ; Drug release ; Erythrocyte ; Fourier transform infrared spectroscopy ; Growth inhibition ; Hemolysis ; Human ; Human cell ; Hydrogen bond ; IC50 ; Magnetic field ; Magnetic separation ; MTT assay ; Particle size ; PEGylation ; PH ; Scanning electron microscopy ; Ultraviolet spectroscopy ; X ray diffraction
  8. Source: Cancers ; Volume 14, Issue 7 , 2022 ; 20726694 (ISSN)
  9. URL: https://www.mdpi.com/2072-6694/14/7/1797