Fabrication and characterization of low-cost, bead-free, durable and hydrophobic electrospun membrane for 3D cell culture

Moghadas, H ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1007/s10544-017-0215-y
  3. Abstract:
  4. This paper reports the fabrication of electrospun polydimethylsiloxane (PDMS) membranes/scaffolds that are suitable for three-dimensional (3D) cell culture. Through modification the ratio between PDMS and polymethylmethacrylate (PMMA) as carrier polymer, we report the possibility of increasing PDMS weight ratio of up to 6 for electrospinning. Increasing the PDMS content increases the fiber diameter, the pore size, and the hydrophobicity. To our best knowledge, this is the first report describing beads-free, durable and portable electrospun membrane with maximum content of PDMS suitable for cell culture applications. To show the proof-of-concept, we successfully cultured epithelial lung cancer cells on these membranes in a static well plate without surface modification. Surprisingly, due to three-dimensional (3D) and hydrophobic nature of the electrospun fibers, cells aggregated into 3D multicellular spheroids. These easily detachable and cost-effective scaffolds with controllable thicknesses and high tensile strength are good candidates for cell-stretching devices, organ-on-a-chip devices, tissue engineering and studies of non-adherent mammalian cancer stem cells. © 2017, Springer Science+Business Media, LLC
  5. Keywords:
  6. Beads-free scaffolds ; Electrospinning cell culture ; Hydrophobic nanofibers ; Spheroid formation ; Cell culture ; Cost effectiveness ; Cost engineering ; Diseases ; Electrospinning ; Hydrophobicity ; Mammals ; Membranes ; Microchannels ; Polydimethylsiloxane ; Pore size ; Scaffolds (biology) ; Silicones ; Spinning (fibers) ; Stem cells ; Surface treatment ; Tensile strength ; Tissue engineering ; Fabrication and characterizations ; High-tensile strength ; Multicellular spheroid ; PDMS membrane ; Polydimethylsiloxane membrane ; Spheroid formation ; Three-dimensional (3d) cell culture ; Threedimensional (3-d) ; Cell engineering ; Baysilon ; Dimeticone ; Poly(methyl methacrylate) ; A-549 cell line ; Artificial membrane ; Cell culture technique ; Chemical phenomena ; Chemistry ; Devices ; Human ; Procedures ; A549 cells ; Cell culture techniques ; Dimethylpolysiloxanes ; Humans ; Hydrophobic and hydrophilic interactions ; Membranes, artificial ; Polymethyl methacrylate
  7. Source: Biomedical Microdevices ; Volume 19, Issue 4 , 2017 ; 13872176 (ISSN)
  8. URL: https://link.springer.com/article/10.1007/s10544-017-0215-y