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An efficient biosurfactant-producing bacterium pseudomonas aeruginosa MR01, isolated from oil excavation areas in south of Iran

Bagheri Lotfabad, T ; Sharif University of Technology | 2009

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  1. Type of Document: Article
  2. DOI: 10.1016/j.colsurfb.2008.11.018
  3. Publisher: 2009
  4. Abstract:
  5. A bacterial strain was isolated and cultured from the oil excavation areas in tropical zone in southern Iran. It was affiliated with Pseudomonas. The biochemical characteristics and partial sequenced 16S rRNA gene of isolate, MR01, was identical to those of cultured representatives of the species Pseudomonas aeruginosa. This bacterium was able to produce a type of biosurfactant with excessive foam-forming properties. Compositional analysis revealed that the extracted biosurfactant was composed of high percentages lipid (∼65%, w/w) and carbohydrate (∼30%, w/w) in addition to a minor fraction of protein (∼4%, w/w). The best production of 2.1 g/l was obtained when the cells were grown on minimal salt medium containing 1.2% (w/v) glucose and 0.1% (w/v) ammonium sulfate supplemented with 0.1% (w/v) isoleucine at 37 °C and 180 rpm after 2 days. The optimum biosurfactant production pH value was found to be 8.0. The MR01 could reduce surface tension to 28 mN/m and emulsified hexadecane up to E24 ≃ 70. The results obtained from time course study indicated that the surface tension reduction and emulsification potential was increased in the same way to cell growth. However, maximum biosurfactant production occurred and established in the stationary growth phase (after 84 h). Fourier Transform Infrared spectrum of extracted biosurfactant indicates the presence of carboxyl, amine, hydroxyl and methoxyl functional groups. Thermogram of biosurfactant demonstrated three sharp endothermic peaks placing between 200 and 280 °C. The core holder flooding experiments demonstrated that the oil recovery efficiencies varied from 23.7% to 27.1% of residual oil. © 2008 Elsevier B.V. All rights reserved
  6. Keywords:
  7. Emulsification ; Oil recovery ; Surface tension ; 16s rrna genes ; Ammonium sulfates ; Bacterial strains ; Biochemical characteristics ; Biosurfactant ; Biosurfactant productions ; Compositional analysis ; Endothermic peaks ; Forming properties ; Fourier transform infrared spectrum ; Growth-phase ; Hexadecane ; Oil recovery efficiencies ; pH values ; Residual oil ; Salt mediums ; Surface tension reductions ; Time course ; Tropical zones ; Amines ; Ammonium compounds ; Biomolecules ; Capillarity ; Carbohydrates ; Cell growth ; Excavation ; Fourier transforms ; Functional groups ; Glucose ; Growth kinetics ; Infrared spectroscopy ; Paraffins ; Surface chemistry ; Surface properties ; Surface waves ; Wetting ; Surface active agents ; Bacterial RNA ; Carbohydrate ; Lipid ; Oil ; Protein ; Ribosome RNA ; Sodium chloride ; Bacterial cell ; Bacterial gene ; Bacterial growth ; Bacterial strain ; Bacterium culture ; Bacterium isolation ; Culture medium ; Emulsion ; Foam ; Gene sequence ; Ion cyclotron resonance mass spectrometry ; Nonhuman ; Oil industry ; Priority journal ; Thermography ; Bacterial Adhesion ; Culture Media ; Hydrogen-Ion Concentration ; Iran ; Oils ; Petroleum ; RNA, Bacterial ; RNA, Ribosomal, 16S ; Soil Microbiology ; Spectroscopy, Fourier Transform Infrared ; Surface-Active Agents ; Bacteria (microorganisms) ; Pseudomonas ; Pseudomonas aeruginosa
  8. Source: Colloids and Surfaces B: Biointerfaces ; Volume 69, Issue 2 , 2009 , Pages 183-193 ; 09277765 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0927776508004190