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Synthesis of green benzamide-decorated UiO-66-NH2 for biomedical applications

Rabiee, N ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1016/j.chemosphere.2022.134359
  3. Publisher: Elsevier Ltd , 2022
  4. Abstract:
  5. Metal-organic frameworks (MOFs) biocompatible systems can host enzymes/bacteria/viruses. Herein we synthesized a series of fatty acid amide hydrolase (FAAH)-decorated UiO-66-NH2 based on Citrus tangerine leaf extract for drug delivery and biosensor applications. Five chemically manipulated FAAH-like benzamides were localized on the UiO-66-NH2 surface with physical interactions. Comprehensive cellular and molecular analyses were conducted on HEK-293, HeLa, HepG2, PC12, MCF-7, and HT-29 cell lines (cytotoxicity assessment after 24 and 48 h). MTT results proved above 95 and 50% relative cell viability in the absence and presence of the drug, respectively. A complete targeted drug-releasing capability of nanocarriers was demonstrated after capping with leaf extract from Citrus tangerine, with a stimuli-responsive effect in acidic media. Targeted delivery was complete to the nucleus and cytoplasm of HT-29 cell, but merely to the cytoplasm of HeLa cell lines. Nanocarrier could be targeted for drug delivery to the cytoplasm of the HeLa cell line and to both the nucleus and cytoplasm of HT-29 cell lines. MOF-based nanocarriers proved authentic in vivo towards kidney and liver tissues with targeted cancerous cells efficiently. Besides, FAAH-like molecules revealed optical biosensor potential with high selectivity (even ˂5 nM LOD) towards ssDNA, sgRNA, and Anti-cas9 proteins. © 2022 Elsevier Ltd
  6. Keywords:
  7. DNA ; FAAH-Like molecules ; Fluorescence biosensor ; MOF ; RNA ; Targeted drug delivery ; Biocompatibility ; Biosensors ; Biosynthesis ; Cell culture ; Citrus fruits ; Controlled drug delivery ; Fatty acids ; Lanthanum compounds ; Medical applications ; Organometallics ; Proteins ; Anti-cas9 protein ; Cell lines ; Fatty acid amide hydrolase ; Fatty acid amide hydrolase-like molecule ; Fluorescence biosensors ; HT 29 cells ; Leaf extracts ; Metalorganic frameworks (MOFs) ; Nanocarriers ; Amides ; Antineoplastic agent ; benzamide ; Citrus tangerine extract ; CRISPR associated endonuclease Cas9 ; Doxorubicin ; Fatty acid amidase ; Metal organic framework ; Nanocarrier ; Nanomaterial ; Unclassified drug ; Benzamide derivative ; Phthalic acid derivative ; Plant extract ; UiO-66 ; Bacterium ; Drug ; Enzyme activity ; Protein ; Cell nucleus ; Cell viability ; Controlled study ; Cytoplasm ; Drug cytotoxicity ; Drug delivery system ; Drug release ; Drug synthesis ; Female ; Fluorescence ; Fourier transform infrared spectroscopy ; Green chemistry ; HEK293 cell line ; HeLa cell line ; Hep-G2 cell line ; HT-29 cell line ; Human ; Human cell ; In vivo study ; Internalization (cell) ; Limit of detection ; MCF-7 cell line ; MTT assay ; Nonhuman ; Tangerine ; Benzamides ; HEK293 cells ; HeLa cells ; Humans ; Metal-Organic frameworks ; Phthalic acids ; Plant extracts
  8. Source: Chemosphere ; Volume 299 , 2022 ; 00456535 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0045653522008529