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Accelerated full-thickness wound healing via sustained bFGF delivery based on a PVA/chitosan/gelatin hydrogel incorporating PCL microspheres

Shamloo, A ; Sharif University of Technology | 2018

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijpharm.2017.12.045
  3. Publisher: Elsevier B.V , 2018
  4. Abstract:
  5. Herein, a hybrid hydrogel/microsphere system is introduced for accelerated wound healing by sustained release of basic fibroblast growth factor (bFGF). The hydrogel is composed of a mixture of PVA, gelatin and chitosan. The double-emulsion-solvent-evaporation method was utilized to obtain microspheres composed of PCL, as the organic phase, and PVA, as the aqueous phase. Subsequently, various in-vitro and in-vivo assays were performed to characterize the system. BSA was used to optimize the release mechanism, and encapsulation efficiency in microspheres, where a combination of 3% (w/v) PCL and 1% (w/v) PVA was found to be the optimum microsphere sample. Incorporation of microspheres within the hydrogel substrate also led to a zero-order release kinetics. Results from SEM images, also represented an average porosity of 54%, and average mean pore size of 35 ± 7 μm for the hydrogel system, and the diameter of 5 ± 2 μm for the microspheres. Moreover, in vivo study including wound healing process, and histological analysis regarding re-epithelization, angiogenesis, inflammation, fibroblast genesis and collagen formation were performed using Hematoxyline-Eosin (H&E) staining, Periodic Acid–Schiff (PAS) staining and Masson's Trichrome staining. In-vivo results represented that sustained delivery of bFGF promoted by biocompatibility of PVA/chitosan/gelatin hydrogel, significantly contribute to accelerated wound healing. © 2017 Elsevier B.V
  6. Keywords:
  7. BFGF ; Drug delivery ; Hydrogel ; Scaffold ; Bovine serum albumin ; Microsphere ; Solvent ; polycaprolactone ; polyester ; polyethylene glycol dimethacrylate hydrogel ; Polyvinyl alcohol ; Angiogenesis ; Animal experiment ; Animal model ; Animal tissue ; Aqueous solution ; Article ; collagen synthesis ; Controlled study ; Drug delivery system ; Emulsion ; Epithelization ; Evaporation ; Fibroblast ; In vitro study ; in vivo study ; Inflammation ; Microencapsulation ; Nonhuman ; Periodic acid Schiff stain ; Priority journal ; Rat ; Scanning electron microscopy ; Sustained drug release ; Administration and dosage ; Animal ; Chemistry ; Delayed release formulation ; Drug effect ; Human ; Wistar rat ; Animals ; Chitosan ; Delayed-Action Preparations ; Emulsions ; Fibroblast growth factor 2 ; Fibroblasts ; Gelatin ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate ; Male ; Microspheres ; particle size ; Polyesters ; Porosity ; Rats ; Rats, Wistar ; Skin ; Wound healing
  8. Source: International Journal of Pharmaceutics ; Volume 537, Issue 1-2 , 2018 , Pages 278-289 ; 03785173 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0378517317311894