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Fabrication and characterization of biaxially electrospun collagen/alginate nanofibers, improved with Rhodotorula mucilaginosa sp. GUMS16 produced exopolysaccharides for wound healing applications

Ashraf, S. S ; Sharif University of Technology | 2022

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijbiomac.2021.11.132
  3. Publisher: Elsevier B.V , 2022
  4. Abstract:
  5. Fabrication of scaffolds with enhanced mechanical properties and desirable cellular compatibility is critical for numerous tissue engineering applications. This study was aimed at fabrication and characterization of a nanofiber skin substitute composed of collagen (Col)/sodium alginate (SA)/ polyethylene oxide (PEO)/Rhodotorula mucilaginosa sp. GUMS16 produced exopolysaccharides (EPS) were prepared using the biaxial electrospinning technique. This study used collagen extracted from the bovine tendon as a natural scaffold, sodium alginate as an absorber of excess wound fluids, and GUMS16 produced exopolysaccharides as an antioxidant. Collagen was characterized using FTIR and EDS analyses. The cross-linked nanofibers were characterized by SEM, FTIR, tensile, contact-angle, swelling test, MTT, and cell attachment techniques. The average diameter of Col nanofiber was 910 ± 89 nm. The Col and Col-SA/PEO non-woven mats' water contact angle measurement was 41.6o and 56.4o, Col/EPS1%, Col/EPS2%, Col-SA/PEO + EPS1%, and Col-SA/PEO + EPS2% were 61.4o, 58.3o, 38.5o, and 50.6o, respectively. Cell viability of more than 100% was shown in Col-SA/PEO + EPS nanofibers. Also, SEM images of cells on nanofiber scaffolds demonstrated that all nanofibers incorporated with GUMS16-produced EPS have good cell growth and proliferation. The acquired results expressed that the GUMS16-produced EPS can be considered a novel biomacromolecule in electrospun fibers that increase cell viability and proliferation. © 2021
  6. Keywords:
  7. Collagen ; Exopolysaccharide ; Tissue engineering ; Wound dressing ; Carbon ; Collagen nanofiber ; Collagen type 1 ; Macrogol ; Nanofiber ; Nitrogen ; Oxygen ; Sodium alginate nanofiber ; Unclassified drug ; Alginic acid ; Biomaterial ; Fungal polysaccharide ; Absorption ; Animal tissue ; Biocompatibility ; Bovine ; Cell adhesion ; Cell migration ; Cell proliferation ; Cell viability ; Contact angle ; Controlled study ; Cross linking ; Cytotoxicity ; Electrospinning ; Energy dispersive X ray spectroscopy ; Fourier transform infrared spectroscopy ; Hydrophilicity ; Nanofabrication ; Nonhuman ; Particle size ; Rhodotorula mucilaginosa ; Scanning electron microscopy ; Tendon ; Tensile strength ; Animal ; Biological dressing ; Chemical phenomena ; Chemistry ; Mechanics ; Spectroscopy ; Alginates ; Animals ; Biocompatible Materials ; Biological Dressings ; Fungal Polysaccharides ; Mechanical Phenomena ; Nanofibers ; Rhodotorula ; Spectrum Analysis ; Wound Healing
  8. Source: International Journal of Biological Macromolecules ; Volume 196 , 2022 , Pages 194-203 ; 01418130 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S014181302102537X