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Structural stability and sustained release of protein from a multilayer nanofiber/nanoparticle composite

Vakilian, S ; Sharif University of Technology | 2015

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijbiomac.2015.01.051
  3. Publisher: Elsevier , 2015
  4. Abstract:
  5. The cellular microenvironment can be engineered through the utilization of various nano-patterns and matrix-loaded bioactive molecules. In this study, a multilayer system of electrospun scaffold containing chitosan nanoparticles was introduced to overcome the common problems of instability and burst release of proteins from nanofibrous scaffolds. Bovine serum albumin (BSA)-loaded chitosan nanoparticles was fabricated based on ionic gelation interaction between chitosan and sodium tripolyphosphate. Suspension electrospinning was employed to fabricate poly-e{open}-caprolacton (PCL) containing protein-loaded chitosan nanoparticles with a core-shell structure. To obtain the desired scaffold mechanical properties with enough elasticity for expansion and contraction, a hybrid mono and multilayer electrospun scaffold was fabricated using PCL containing protein-loaded chitosan nanoparticles and poly-. l-lactic acid (PLLA). According to the BSA release profile, the multi-layered structure of nanofibers with two barrier layers provided a programmable release pattern of the loaded protein. Moreover, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and circular dichroism spectra results showed that the electrospinning process had no significant effect on the primary and secondary structure of the protein. The results indicated a desirable biocompatibility and mechanical cues of the multilayer nanofibrous scaffolds supporting structural stability and controlled release of the protein, which can offer diverse applications in hollow organ tissue engineering
  6. Keywords:
  7. Multilayer scaffold ; Protein structural stability ; Sustained release ; Bovine serum albumin ; Chitosan ; Chitosan nanoparticle ; Molecular scaffold ; Polycaprolactone ; Polylactic acid ; Protein ; Tripolyphosphate ; Nanoparticle ; Amino acid sequence ; Biocompatibility ; Controlled study ; Elasticity ; Electrospinning ; Gelation ; Human cell ; Nanofabrication ; Polyacrylamide gel electrophoresis ; Protein secondary structure ; Protein secretion ; Protein stability ; Protein structure ; Tissue engineering ; Cell motion ; Chemistry ; Cytology ; Delayed release formulation ; Mechanics ; Mesenchymal stroma cell ; Metabolism ; Photon correlation spectroscopy ; Ultrastructure ; Bovinae ; Animals ; Cattle ; Cell Movement ; Cell Proliferation ; Cell Survival ; Circular Dichroism ; Delayed-Action Preparations ; Dynamic Light Scattering ; Electrophoresis, Polyacrylamide Gel ; Humans ; Lactic Acid ; Mechanical Phenomena ; Mesenchymal Stromal Cells ; Molecular Weight ; Nanofibers ; Nanoparticles ; Polyesters ; Polymers ; Protein Structure, Secondary ; Serum Albumin, Bovine ; Tensile Strength ; Thermogravimetry ; Tissue Scaffolds
  8. Source: International Journal of Biological Macromolecules ; Volume 75 , April , 2015 , Pages 248-257 ; 01418130 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0141813015000628