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Cell toxicity of superparamagnetic iron oxide nanoparticles

Mahmoudi, M ; Sharif University of Technology | 2009

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  1. Type of Document: Article
  2. DOI: 10.1016/j.jcis.2009.04.046
  3. Publisher: 2009
  4. Abstract:
  5. The performance of nanoparticles for biomedical applications is often assessed by their narrow size distribution, suitable magnetic saturation and low toxicity effects. In this work, superparamagnetic iron oxide nanoparticles (SPIONs) with different size, shape and saturation magnetization levels were synthesized via a co-precipitation technique using ferrous salts with a Fe3+/Fe2+ mole ratio equal to 2. A parametric study is conducted, based on a uniform design-of-experiments methodology and a critical polymer/iron mass ratio (r-ratio) for obtaining SPION with narrow size distribution, suitable magnetic saturation, and optimum biocompatibility is identified. Polyvinyl alcohol (PVA) has been used as the nanoparticle coating material, owing to its low toxicity. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is used to investigate the cell biocompatibility/toxicity effects of the samples. From the MTT assay results, it is observed that the biocompatibility of the nanoparticles, based on cell viabilities, can be enhanced by increasing the r-ratio, regardless of the stirring rate. This effect is mainly due to the growth of the particle hydrodynamic size, causing lower cell toxicity effects. © 2009 Elsevier Inc. All rights reserved
  6. Keywords:
  7. Cell toxicity ; Magnetite ; MTT assay ; Polymeric nanocomposite ; Superparamagnetic nanoparticles ; Biomedical applications ; Different sizes ; Ferrous salts ; Low toxicity ; Magnetic saturation ; Mass ratio ; Mole ratio ; MTT assay ; Nanoparticle coatings ; Narrow size distributions ; Parametric study ; Particle hydrodynamics ; R ratio ; Stirring rates ; Superparamagnetic iron oxide nanoparticles ; Superparamagnetic nanoparticles ; Uniform Design ; Biocompatibility ; Fluid dynamics ; Iron ; Iron oxides ; Nanoparticles ; Oxide minerals ; Polymers ; Precipitation (chemical) ; Salts ; Size distribution ; Superparamagnetism ; Saturation magnetization ; 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide ; polymer ; polyvinyl alcohol ; Superparamagnetic iron oxide nanoparticle ; Animal cell ; Article ; Biomedicine ; cell assay ; Cell viability ; Controlled study ; Cytotoxicity ; Hydrodynamics ; Magnetism ; Material coating ; Methodology ; Mouse ; Nonhuman ; Parametric test ; Particle size ; Precipitation ; Priority journal ; Animals ; Biocompatible Materials ; Cell Line ; Cell Survival ; Ferric Compounds ; Ferrous Compounds ; Humans ; Magnetics ; Nanoparticles ; Polyvinyl Alcohol
  8. Source: Journal of Colloid and Interface Science ; Volume 336, Issue 2 , 2009 , Pages 510-518 ; 00219797 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0021979709004664