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Preparation, physicochemical properties, in vitro evaluation and release behavior of cephalexin-loaded niosomes

Ghafelehbashi, R ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1016/j.ijpharm.2019.118580
  3. Publisher: Elsevier B.V , 2019
  4. Abstract:
  5. In this study, optimized cephalexin-loaded niosomal formulations based on span 60 and tween 60 were prepared as a promising drug carrier system. The niosomal formulations were characterized using a series of techniques such as scanning electron microscopy, Fourier transformed infrared spectroscopy, dynamic light scattering, and zeta potential measurement. The size and drug encapsulation efficiency are determined by the type and composition of surfactant. The developed niosomal formulations showed great storage stability up to 30 days with low change in size and drug entrapment during the storage, making them potential candidates for real applications. Moreover, the prepared niosomes showed negligible cytotoxicity for HepG2 cells, measured by MTT assay. The antibacterial properties of cephalexin-loaded niosome were investigated using S. aureus and E. coli as gram-positive and gram-negative bacteria, respectively. The results showed that the encapsulation of antibiotic drug in niosomal formulation could enhance the antibacterial efficiency of the drug, where the minimum inhibitory concentration was droped from 8 µg/mL (cephalexin) to 4 µg/mL (cephalexin-loaded niosome) and from 4 µg/mL (cephalexin) to 1 µg/mL (cephalexin-loaded niosome) against E. coli and S. aureus, respectively. The findings of our study show that the improvement of cephalexin bioavailability and prolonged drug release profile could be obtained by niosomal formulation as a favorable antibiotic drug delivery system. © 2019 Elsevier B.V
  6. Keywords:
  7. Antibacterial ; Cephalexin ; Cytotoxicity ; Drug delivery ; Niosome ; Cefalexin ; Polysorbate 60 ; Sorbitan stearate ; Surfactant ; Antiinfective agent ; Liposome ; Antibacterial activity ; Article ; Clinical evaluation ; Drug bioavailability ; Drug delivery system ; Drug efficacy ; Drug formulation ; Drug storage ; Drug synthesis ; Escherichia coli ; Fourier transform spectroscopy ; Hep-G2 cell line ; Human ; Human cell ; In vitro study ; Minimum inhibitory concentration ; MTT assay ; Nanoencapsulation ; Particle size ; Photon correlation spectroscopy ; Physical chemistry ; Priority journal ; Scanning electron microscopy ; Staphylococcus aureus ; Sustained drug release ; Zeta potential ; Chemistry ; Drug effect ; Drug release ; Growth, development and aging ; Anti-Bacterial Agents ; Cell Survival ; Drug Liberation ; Drug Stability ; Hep G2 Cells ; Humans ; Liposomes
  8. Source: International Journal of Pharmaceutics ; Volume 569 , 2019 ; 03785173 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0378517319306246