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Design of experiment, preparation, and in vitro biological assessment of human amniotic membrane extract loaded nanoparticles

Shabani, A ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.2174/1389201020666191019122130
  3. Publisher: Bentham Science Publishers , 2020
  4. Abstract:
  5. Background: Human amniotic membrane grafting could be potentially useful in ocular surface complications due to tissue similarity and the presence of factors that reduce inflammation, vascu-larization, and scarring. However, considerations like donor-derived infectious risk and the requirement of an invasive surgery limit the clinical application of such treatments. Moreover, the quick depletion of bioactive factors after grafting reduces the efficacy of treatments. Therefore, in the current study, the possibility of nano delivery of the bioactive factors extracted from the human amniotic membrane to the ocular surface was investigated. Materials and Methods: Nanoparticles were prepared using polyelectrolyte complexation from chitosan and dextran sulfate. The effect of polymer ratio, pH, and the amount of extract on particle size and encapsulation efficacy were studied using Box-Behnken response surface methodology. Results: The optimum condition was obtained as follows: 4.9:1 ratio of dextran sulfate to chitosan, 600 µL amniotic membrane extract, and pH of 6. The prepared nanoparticles had an average size of 213 nm with 77% encapsulation efficacy. In the release test, after 10 days, approximately 50% of entrapped bioactive proteins were released from the nanocarriers in a controlled manner. Biological activity assessment on endothelial cells revealed amniotic membrane extract loaded nanoparticles had a longer and significant increase in anti-angiogenic effect when compared to the control. Conclusion: Our data elucidate the ability of nanotechnology in ocular targeted nano delivery of bioactive compounds. © 2020 Bentham Science Publishers
  6. Keywords:
  7. Amniotic membrane extract ; Chitosan ; Dextran sulfate ; Nanocarriers ; Nanodelivery ; Ocular disease ; Amniotic membrane extract loaded nanoparticle ; Calcium chloride ; Carbon 14 ; Chitosan ; Chitosan nanoparticle ; Dextran sulfate ; Drug carrier ; polymer ; Sulfate ; Unclassified drug ; Angiogenesis inhibitor ; Nanoparticle ; Tissue extract ; Amnion ; Antiangiogenic activity ; Biological activity ; Cell proliferation ; Cell viability ; Complex formation ; Controlled study ; Depolymerization ; Dispersity ; Drug delivery system ; Drug release ; Drug stability ; Endothelium cell ; Enzyme linked immunosorbent assay ; Experimental design ; Gel permeation chromatography ; Infrared spectroscopy ; Light scattering ; MTT assay ; Nanoencapsulation ; Nanotechnology ; Photon correlation spectroscopy ; Physical chemistry ; Response surface method ; Scanning electron microscopy ; Surface charge ; Surface property ; Sustained drug release ; Zeta potential ; Amnion ; Cell culture ; Chemistry ; Drug effect ; Drug formulation ; Isolation and purification ; Methodology ; Umbilical vein endothelial cell ; Amnion ; Angiogenesis Inhibitors ; Cell Proliferation ; Cells, Cultured ; Chitosan ; Drug Carriers ; Drug Compounding ; Drug Liberation ; Human Umbilical Vein Endothelial Cells ; Humans ; Nanoparticles ; Particle size ; Research Design ; Surface Properties ; Tissue Extracts
  8. Source: Current Pharmaceutical Biotechnology ; Volume 21, Issue 3 , 2020 , Pages 256-267
  9. URL: https://www.eurekaselect.com/175895/article