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Simultaneous controlled release of 5-FU, DOX and PTX from chitosan/PLA/5-FU/g-C3N4-DOX/g-C3N4-PTX triaxial nanofibers for breast cancer treatment in vitro

Habibi Jouybari, M ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1016/j.colsurfb.2019.04.026
  3. Publisher: Elsevier B.V , 2019
  4. Abstract:
  5. In the present study, the tri-layer nanofibers were synthesized via triaxial electrospinning process to control the sustained delivery of Doxorubicin (DOX), Paclitaxel (PTX) and 5- fluorouracil (5-FU) anticancer drugs from nanofibers. The 5-FU molecules were incorporated into the core solution (chitosan/polyvinyl alcohol (CS/PVA)) to fabricate the CS/PVA/5-FU inner layer of nanofibers. The intermediate layer was prepared from poly(lactic acid)/chitosan (PLA/CS) nanofibers. The DOX and PTX molecules were initially loaded into the g-C3N4 nanosheets and following were incorporated into the PLA/CS solution to fabricate the outer layer of nanofibers. The synthesized nanosheets and nanofibers were characterized using XRD, SEM, TEM and UV–vis analysis. The PLA/PVA/CS/FU/g-C3N4/DOX/PTX single layer nanofibers were also synthesized via electrospinning method. The drug loading efficiency, degradation rate and anticancer drugs release profiles from single layer and tri-layer nanofibers were investigated under various intermediate and shell layer thicknesses. The pharmacokinetic studies were performed to understand the drugs release mechanism from nanofibers. The cell viability and cell attachment of drug loaded single layer and tri-layer nanofibers toward the MCF-7 breast cancer cells were examined to achieve an optimum nanofibrous formulation for the breast cancer treatment. The obtained results revealed the high activity of tri-layer nanofibers for the breast cancer cells killing. © 2019
  6. Keywords:
  7. 5-Flouracil ; C3N4 loaded-tri-layer nanofibers ; Chitosan ; Paclitaxel ; Cells ; Cytology ; Degradation ; Diseases ; Drug products ; Electrospinning ; Molecules ; Nanofibers ; Nanosheets ; Targeted drug delivery ; Breast cancer treatment ; Drug-loading efficiency ; Electrospinning process ; MCF-7 breast cancer cells ; Tri layers ; Controlled drug delivery ; Carbon ; Drug carrier ; Nanoparticle ; Nitrogen ; Polyvinyl alcohol ; Cyanogen ; Nanofiber ; Nitrile ; Polyester ; Polylactide ; Antineoplastic activity ; Article ; Breast cancer ; Cancer cell ; Cell adhesion ; Cell killing ; Cell viability ; Controlled drug release ; Controlled study ; Drug degradation ; Drug delivery system ; Drug formulation ; Drug mechanism ; Drug release ; Drug solubility ; Human ; In vitro study ; MCF-7 cell line ; Priority journal ; Scanning electron microscopy ; Transmission electron microscopy ; Ultraviolet visible spectrophotometry ; X ray diffraction ; Breast tumor ; Chemistry ; Delayed release formulation ; Drug effect ; Pharmacokinetics ; Pharmacology ; Ultrastructure ; Breast Neoplasms ; Cell Adhesion ; Cell Survival ; Delayed-Action Preparations ; Doxorubicin ; Drug Liberation ; Female ; Fluorouracil ; Graphite ; Humans ; Kinetics ; MCF-7 Cells ; Nitriles ; Polyesters ; X-Ray Diffraction
  8. Source: Colloids and Surfaces B: Biointerfaces ; Volume 179 , 2019 , Pages 495-504 ; 09277765 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S0927776519302498